RT97: a marker for capsaicin-insensitive sensory endings in the rat skin

Holger Sann, Peter W. McCarthy, G. Jancsó, Friedrich Karl Pierau

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The mouse monoclonal antibody RT97, which recognises the 200-kDa neurofilament subunit in its phosphorylated form, selectively labels the somata of sensory A-fibres (large light cells) in the dorsal root ganglion of the rat. We have tested the hypothesis that this antibody also visualises large diameter sensory fibres and their end structures in peripheral tissue, in particular in the skin. RT97 immunoreactivity is found in endings that are known to be served by myelinated afferent fibres, including Meissner-like endings, Merkel discs, hair follicle receptors, Pacinian corpuscles and free nerve endings. RT97 immunoreactivity has not, however, been observed in endings of presumably unmyelinated sensory fibres (intraepidermal fibres immunoreactive for substance P and calcitonin gene-related peptide) or in sympathetic fibres innervating sweat glands and blood vessels. In addition, neither systemic (100-150 mg/kg as adults) nor perineural capsaicin pre-treatment affects RT97 immunoreactivity in the skin. The data indicate that RT97 is a useful marker in the study of the capsaicin-insensitive sensory innervation of the skin and possibly other peripheral organs.

Original languageEnglish
Pages (from-to)155-161
Number of pages7
JournalCell and Tissue Research
Volume282
Issue number1
DOIs
Publication statusPublished - Oct 1995

Fingerprint

Capsaicin
Rats
Skin
Fibers
Pacinian Corpuscles
Adrenergic Fibers
Myelinated Nerve Fibers
Sweat Glands
Nerve Endings
Hair Follicle
Calcitonin Gene-Related Peptide
Spinal Ganglia
Carisoprodol
Substance P
Blood Vessels
Monoclonal Antibodies
Antibodies
Blood vessels
Labels
Tissue

Keywords

  • Capsaicin
  • Immunohistochemistry
  • Neurofilament
  • Primary afferent fibres
  • Rat (Wistar)
  • Skin

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

RT97 : a marker for capsaicin-insensitive sensory endings in the rat skin. / Sann, Holger; McCarthy, Peter W.; Jancsó, G.; Pierau, Friedrich Karl.

In: Cell and Tissue Research, Vol. 282, No. 1, 10.1995, p. 155-161.

Research output: Contribution to journalArticle

Sann, Holger ; McCarthy, Peter W. ; Jancsó, G. ; Pierau, Friedrich Karl. / RT97 : a marker for capsaicin-insensitive sensory endings in the rat skin. In: Cell and Tissue Research. 1995 ; Vol. 282, No. 1. pp. 155-161.
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