RT-PCR Analysis and Stress Response Capacity of Transgenic gshl-Poplar Clones (Populus x canescens) in Response to Paraquat Exposure

András Bittsánszky, Gábor Gyulai, Jozsef Kiss, Zoltán SzabO, Richárd Lágler, Zoltán Toth, Lászlo Heszky, Mervyn Humphreys, Zsolt Csintalan, Gábor Gullnerd, Tamás Kőmíves, Heinz Rennenberg

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Stress response capacity (Fv/Fm at 690 nm and F690/F735 at Fmax) of untransformed hybrid poplar, Populus × canescens (P. tremula × P. alba), and two transgenic lines overexpressing γ-ECS (γ-glutamylcysteine synthetase) either in the cytosol (cyt-ECS) or in the chloroplast (chl-ECS) was studied in response to the herbicide paraquat (4.0 × 10-9 to 4.0 × 10-6 m) for 21 days. Significant differences at sublethal (4.0 × 10-7 m) and bleaching (4.0 × 10-6 m) concentrations of paraquat were observed with about a two-fold and eight-fold decrease in the photosynthetic activity (Fv/Fm at 690 nm and F690/F735 at Fmax), respectively. None of the gshI transgenic lines (cyt-ECS, chl-ECS) with elevated GSH content exhibited significant tolerance to paraquat. Semiquantitative RT-PCR of the cyt-ECS clone was used for gene expression analysis of the nuclear encoded rbcS gene and the stress responsive gst gene. Expression of the constitutively expressed 26SrRNA ribosomal gene was probed as a control for all RT-PCR reactions. The relative intensities of gene expressions normalized to the level of 26SrRNA intensity showed a 50% decrease in the nuclear encoded rbcS expression and a 120% increase in the stress responsive gst gene expression of the paraquat treated (4.0 × 10-7 m) samples of the transgenic poplar line (cyt-ECS).

Original languageEnglish
Pages (from-to)699-703
Number of pages5
JournalZeitschrift fur naturforschung - section c journal of biosciences
Volume61
Issue number9-10
DOIs
Publication statusPublished - Oct 2006

Keywords

  • Paraquat Stress
  • Populus × canescens
  • chl-ECS (lgl6)
  • cyt-ECS (ggs11)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'RT-PCR Analysis and Stress Response Capacity of Transgenic gshl-Poplar Clones (Populus x canescens) in Response to Paraquat Exposure'. Together they form a unique fingerprint.

  • Cite this