RP-HPLC separation of polyamines after automatic FMOC-Cl derivatization and precolumn sample clean-up using column switching

T. Bartók, G. Borcsok, F. Sagi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A fully automated RP-HPLC procedure including precolumn derivatizaton and sample clean-up was developed for the separation and determination of polyamines in plant tissue. The crude plant extract was derivatized with FMOC-Cl during 3 min without a prechromatographic purification step. The sample matrix, the reagent excess and most of the reaction by-products were effectively removed by a simple end-cut technique using column switching. The FMOC-polyamines were separated through an ODS microbore column with good resolution and detected with a fluorescence detector. The detection limit was less than 40 femtomoles for all analysed FMOC-polyamines.

Original languageEnglish
Pages (from-to)777-790
Number of pages14
JournalJournal of Liquid Chromatography
Volume15
Issue number5
Publication statusPublished - 1992

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Polyamines
High Pressure Liquid Chromatography
Plant Extracts
Complex Mixtures
Purification
Byproducts
Limit of Detection
Fluorescence
Tissue
Detectors
1-(9-fluorenyl)methyl chloroformate

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

RP-HPLC separation of polyamines after automatic FMOC-Cl derivatization and precolumn sample clean-up using column switching. / Bartók, T.; Borcsok, G.; Sagi, F.

In: Journal of Liquid Chromatography, Vol. 15, No. 5, 1992, p. 777-790.

Research output: Contribution to journalArticle

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