Rosuvastatin induces delayed preconditioning against L-glutamate excitotoxicity in cultured cortical neurons

F. Domoki, Béla Kis, Tamás Gáspár, James A. Snipes, F. Bari, David W. Busija

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We tested whether rosuvastatin (RST) protected against excitotoxic neuronal cell death in rat primary cortical neuronal cultures. l-Glutamate (200μM, 1h) reduced neuronal viability (% of naive controls, mean±SEM, n=8-32, *p2+ and reduced increases in superoxide anion levels following glutamate exposure. Manganese superoxide dismutase (SOD), copper-zinc SOD, catalase, and reduced glutathione levels were unaffected by RST pretreatment. In contrast, acute, one time RST application did not affect either baseline or l-glutamate-induced increases in superoxide levels. In summary, three-day RST pretreatment induces resistance to the excitotoxic effect of l-glutamate in cultured neurons apparently by a mechanism that is independent of 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase inhibition. The delayed neuroprotection by RST against excitotoxicity does not involve sustained mitochondrial depolarization or superoxide anion production as initiating events, although it is associated with reduced Ca2+ influx and superoxide anion production upon l-glutamate challenge.

Original languageEnglish
Pages (from-to)404-409
Number of pages6
JournalNeurochemistry International
Volume56
Issue number3
DOIs
Publication statusPublished - Feb 2010

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Glutamic Acid
Superoxides
Neurons
Superoxide Dismutase
Catalase
Glutathione
Zinc
Copper
Oxidoreductases
Cell Death
Rosuvastatin Calcium

Keywords

  • Cell culture
  • Intracellular calcium
  • Neuroprotection
  • Reactive oxygen species
  • Statins

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Rosuvastatin induces delayed preconditioning against L-glutamate excitotoxicity in cultured cortical neurons. / Domoki, F.; Kis, Béla; Gáspár, Tamás; Snipes, James A.; Bari, F.; Busija, David W.

In: Neurochemistry International, Vol. 56, No. 3, 02.2010, p. 404-409.

Research output: Contribution to journalArticle

Domoki, F. ; Kis, Béla ; Gáspár, Tamás ; Snipes, James A. ; Bari, F. ; Busija, David W. / Rosuvastatin induces delayed preconditioning against L-glutamate excitotoxicity in cultured cortical neurons. In: Neurochemistry International. 2010 ; Vol. 56, No. 3. pp. 404-409.
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AU - Busija, David W.

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