Role of the vitamin E model compound Trolox in the prevention of Cr(VI)-induced cellular damage

Borut Poljšak, Zoltán Gazdag, Miklós Pesti, Metka Filipič, Stefan Fujs, Nelli Farkas, Stojan Plesničar, Peter Raspor

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Given the wide industrial use of chromium (Cr) and its environmental contamination, chromium represents a risk to humans exposed to the metal. Considering that Cr(VI) is a potent oxidizing agent that increases intracellular oxidation and DNA damage, it would be worth considering the pretreatment of cells with antioxidants as a means of preventing Cr(VI)-induced toxicity. The objective of this study was to pretreat yeast cells with the water-soluble vitamin E analogue Trolox in an effort to increase cell tolerance against reactive chromium and reactive oxygen species formed during Cr(VI) reduction. Results revealed a decrease in Cr(VI)-induced cytotoxicity and mitotic gene conversions in Trolox-pretreated cells. The protective effect of Trolox in Cr(VI) induced genotoxicity was confirmed also with the prokaryotic Salmonella typhimurium SOS/umu test. Pretreatment of cells with Trolox (1) increased total Cr bioaccumulation, (2) decreased Cr(VI)-induced intracellular oxidation, (3) decreased Cr(V) persistence and (4) increased OH formation in yeast extracts. These findings might be useful in directing future investigations concerning the use of Trolox as a human antioxidant supplement, and in clinical applications related to Cr-induced genotoxicity in occupational and environmental situations where chromium is a problem.

Original languageEnglish
Pages (from-to)141-157
Number of pages17
JournalToxicological and Environmental Chemistry
Volume88
Issue number1
DOIs
Publication statusPublished - Jan 1 2006

Keywords

  • Antioxidants
  • Cr(VI)
  • Free radicals
  • Trolox
  • Yeast Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution
  • Health, Toxicology and Mutagenesis

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