Role of the time factor in the development of hormonal imprinting of insulin receptors in Chang liver cell cultures.

G. Csaba, E. Bohdaneczky, P. Kovács

Research output: Contribution to journalArticle

Abstract

Chang liver cells were pretreated with insulin for 5, 10, 20 and 60 minutes, and 4 and 24 hours after the treatment the binding of FITC-labelled hormone was measured. Hormone binding decreased after 5 minutes treatment, while longer treatments the binding increased considerably. The binding of hormone, at 4 hours investigation, to the nuclear membrane increased more markedly than in plasma membrane, indicating the important role of the imprinting of nuclear membrane. At 24 hours, the increased binding capacity is more characteristic for the plasma membrane.

Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalActa Physiologica Hungarica
Volume72
Issue number1
Publication statusPublished - 1988

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Insulin Receptor
Cell Culture Techniques
Nuclear Envelope
Hormones
Liver
Cell Membrane
Fluorescein-5-isothiocyanate
Insulin

ASJC Scopus subject areas

  • Physiology

Cite this

Role of the time factor in the development of hormonal imprinting of insulin receptors in Chang liver cell cultures. / Csaba, G.; Bohdaneczky, E.; Kovács, P.

In: Acta Physiologica Hungarica, Vol. 72, No. 1, 1988, p. 35-40.

Research output: Contribution to journalArticle

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