Role of the Na+/Ca2+ exchanger in calcium homeostasis and human sperm motility regulation

Z. Krasznai, Zoárd Tibor Krasznai, Masaaki Morisawa, Zsuzsa Kassai Bazsáné, Z. Hernádi, Zsolt Fazekas, L. Trón, K. Goda, T. Márián

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A number of cell functions, such as flagellar beating, swimming velocity, acrosome reaction, etc., are triggered by a Ca2+ influx across the cell membrane. For appropriate physiological functions, the motile human sperm maintains the intracellular free calcium concentration ([Ca2+] i) at a submicromolar level. The objective of this study was to determine the role of the Na+/Ca2+ exchanger (NCX) in the maintenance of [Ca2+]i in human spermatozoa. Spermatozoa maintained in extracellular medium containing ≥1 μM Ca2+ exhibited motility similar to that of the control. In addition to several calcium transport mechanisms described earlier, we provide evidence that the NCX plays a crucial role in the maintenance of [Ca2+]i. Three chemically unrelated inhibitors of the NCX (bepridil, DCB (3′,4′- dichlorobenzamil hydrochloride), and KB-R7943) all blocked human sperm motility in a dose and incubation time dependent manner. The IC50 values for bepridil, DCB, and KB-R7943 were 16.2, 9.8, and 5.3 μM, respectively. The treatment with the above-mentioned blockers resulted in an elevated [Ca 2+]i and a decreased [Na+]i. The store-operated calcium channel (SOCC) inhibitor SKF 96365 also blocked the sperm motility (IC50 = 2.44 μM). The presence of the NCX antigen in the human spermatozoa was proven by flow cytometry, confocal laser scanning microscopy, and immunoblotting techniques. Calcium homeostasis of human spermatozoa is maintained by several transport proteins among which the SOCC and the NCX may play a major role.

Original languageEnglish
Pages (from-to)66-76
Number of pages11
JournalCell Motility and the Cytoskeleton
Volume63
Issue number2
DOIs
Publication statusPublished - Feb 2006

Fingerprint

Sperm Motility
Spermatozoa
Homeostasis
Calcium
Bepridil
1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole
Calcium Channels
Inhibitory Concentration 50
Maintenance
Acrosome Reaction
Immunoblotting
Confocal Microscopy
Carrier Proteins
Flow Cytometry
Cell Count
Cell Membrane
Antigens
2-(2-(4-(4-nitrobenzyloxy)phenyl)ethyl)isothiourea methanesulfonate

Keywords

  • Bepridil
  • Ca homeostasis
  • Human sperm motility
  • Intracellular calcium and membrane potential
  • Na/Ca exchanger

ASJC Scopus subject areas

  • Cell Biology

Cite this

Role of the Na+/Ca2+ exchanger in calcium homeostasis and human sperm motility regulation. / Krasznai, Z.; Krasznai, Zoárd Tibor; Morisawa, Masaaki; Bazsáné, Zsuzsa Kassai; Hernádi, Z.; Fazekas, Zsolt; Trón, L.; Goda, K.; Márián, T.

In: Cell Motility and the Cytoskeleton, Vol. 63, No. 2, 02.2006, p. 66-76.

Research output: Contribution to journalArticle

Krasznai, Z. ; Krasznai, Zoárd Tibor ; Morisawa, Masaaki ; Bazsáné, Zsuzsa Kassai ; Hernádi, Z. ; Fazekas, Zsolt ; Trón, L. ; Goda, K. ; Márián, T. / Role of the Na+/Ca2+ exchanger in calcium homeostasis and human sperm motility regulation. In: Cell Motility and the Cytoskeleton. 2006 ; Vol. 63, No. 2. pp. 66-76.
@article{5ae9e349affd414a8fcc424121dcd161,
title = "Role of the Na+/Ca2+ exchanger in calcium homeostasis and human sperm motility regulation",
abstract = "A number of cell functions, such as flagellar beating, swimming velocity, acrosome reaction, etc., are triggered by a Ca2+ influx across the cell membrane. For appropriate physiological functions, the motile human sperm maintains the intracellular free calcium concentration ([Ca2+] i) at a submicromolar level. The objective of this study was to determine the role of the Na+/Ca2+ exchanger (NCX) in the maintenance of [Ca2+]i in human spermatozoa. Spermatozoa maintained in extracellular medium containing ≥1 μM Ca2+ exhibited motility similar to that of the control. In addition to several calcium transport mechanisms described earlier, we provide evidence that the NCX plays a crucial role in the maintenance of [Ca2+]i. Three chemically unrelated inhibitors of the NCX (bepridil, DCB (3′,4′- dichlorobenzamil hydrochloride), and KB-R7943) all blocked human sperm motility in a dose and incubation time dependent manner. The IC50 values for bepridil, DCB, and KB-R7943 were 16.2, 9.8, and 5.3 μM, respectively. The treatment with the above-mentioned blockers resulted in an elevated [Ca 2+]i and a decreased [Na+]i. The store-operated calcium channel (SOCC) inhibitor SKF 96365 also blocked the sperm motility (IC50 = 2.44 μM). The presence of the NCX antigen in the human spermatozoa was proven by flow cytometry, confocal laser scanning microscopy, and immunoblotting techniques. Calcium homeostasis of human spermatozoa is maintained by several transport proteins among which the SOCC and the NCX may play a major role.",
keywords = "Bepridil, Ca homeostasis, Human sperm motility, Intracellular calcium and membrane potential, Na/Ca exchanger",
author = "Z. Krasznai and Krasznai, {Zo{\'a}rd Tibor} and Masaaki Morisawa and Bazs{\'a}n{\'e}, {Zsuzsa Kassai} and Z. Hern{\'a}di and Zsolt Fazekas and L. Tr{\'o}n and K. Goda and T. M{\'a}ri{\'a}n",
year = "2006",
month = "2",
doi = "10.1002/cm.20108",
language = "English",
volume = "63",
pages = "66--76",
journal = "Cytoskeleton",
issn = "1949-3584",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Role of the Na+/Ca2+ exchanger in calcium homeostasis and human sperm motility regulation

AU - Krasznai, Z.

AU - Krasznai, Zoárd Tibor

AU - Morisawa, Masaaki

AU - Bazsáné, Zsuzsa Kassai

AU - Hernádi, Z.

AU - Fazekas, Zsolt

AU - Trón, L.

AU - Goda, K.

AU - Márián, T.

PY - 2006/2

Y1 - 2006/2

N2 - A number of cell functions, such as flagellar beating, swimming velocity, acrosome reaction, etc., are triggered by a Ca2+ influx across the cell membrane. For appropriate physiological functions, the motile human sperm maintains the intracellular free calcium concentration ([Ca2+] i) at a submicromolar level. The objective of this study was to determine the role of the Na+/Ca2+ exchanger (NCX) in the maintenance of [Ca2+]i in human spermatozoa. Spermatozoa maintained in extracellular medium containing ≥1 μM Ca2+ exhibited motility similar to that of the control. In addition to several calcium transport mechanisms described earlier, we provide evidence that the NCX plays a crucial role in the maintenance of [Ca2+]i. Three chemically unrelated inhibitors of the NCX (bepridil, DCB (3′,4′- dichlorobenzamil hydrochloride), and KB-R7943) all blocked human sperm motility in a dose and incubation time dependent manner. The IC50 values for bepridil, DCB, and KB-R7943 were 16.2, 9.8, and 5.3 μM, respectively. The treatment with the above-mentioned blockers resulted in an elevated [Ca 2+]i and a decreased [Na+]i. The store-operated calcium channel (SOCC) inhibitor SKF 96365 also blocked the sperm motility (IC50 = 2.44 μM). The presence of the NCX antigen in the human spermatozoa was proven by flow cytometry, confocal laser scanning microscopy, and immunoblotting techniques. Calcium homeostasis of human spermatozoa is maintained by several transport proteins among which the SOCC and the NCX may play a major role.

AB - A number of cell functions, such as flagellar beating, swimming velocity, acrosome reaction, etc., are triggered by a Ca2+ influx across the cell membrane. For appropriate physiological functions, the motile human sperm maintains the intracellular free calcium concentration ([Ca2+] i) at a submicromolar level. The objective of this study was to determine the role of the Na+/Ca2+ exchanger (NCX) in the maintenance of [Ca2+]i in human spermatozoa. Spermatozoa maintained in extracellular medium containing ≥1 μM Ca2+ exhibited motility similar to that of the control. In addition to several calcium transport mechanisms described earlier, we provide evidence that the NCX plays a crucial role in the maintenance of [Ca2+]i. Three chemically unrelated inhibitors of the NCX (bepridil, DCB (3′,4′- dichlorobenzamil hydrochloride), and KB-R7943) all blocked human sperm motility in a dose and incubation time dependent manner. The IC50 values for bepridil, DCB, and KB-R7943 were 16.2, 9.8, and 5.3 μM, respectively. The treatment with the above-mentioned blockers resulted in an elevated [Ca 2+]i and a decreased [Na+]i. The store-operated calcium channel (SOCC) inhibitor SKF 96365 also blocked the sperm motility (IC50 = 2.44 μM). The presence of the NCX antigen in the human spermatozoa was proven by flow cytometry, confocal laser scanning microscopy, and immunoblotting techniques. Calcium homeostasis of human spermatozoa is maintained by several transport proteins among which the SOCC and the NCX may play a major role.

KW - Bepridil

KW - Ca homeostasis

KW - Human sperm motility

KW - Intracellular calcium and membrane potential

KW - Na/Ca exchanger

UR - http://www.scopus.com/inward/record.url?scp=32544450496&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=32544450496&partnerID=8YFLogxK

U2 - 10.1002/cm.20108

DO - 10.1002/cm.20108

M3 - Article

VL - 63

SP - 66

EP - 76

JO - Cytoskeleton

JF - Cytoskeleton

SN - 1949-3584

IS - 2

ER -