Role of the N-terminal transmembrane domain in the endo-lysosomal targeting and function of the human ABCB6 protein

Katalin Kiss, Nora Kucsma, Anna Brozik, Gabor E. Tusnady, Ptissam Bergam, Guillaume Van Niel, Gergely Szakacs

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

ATP-binding cassette, subfamily B (ABCB) 6 is a homodimeric ATP-binding cassette (ABC) transporter present in the plasma membrane and in the intracellular organelles. The intracellular localization of ABCB6 has been a matter of debate, as it has been suggested to reside in the mitochondria and the endo-lysosomal system. Using a variety of imaging modalities, including confocal microscopy and EM, we confirm the endo-lysosomal localization of ABCB6 and show that the protein is internalized from the plasma membrane through endocytosis, to be distributed to multivesicular bodies and lysosomes. In addition to the canonical nucleotide-binding domain (NBD) and transmembrane domain (TMD), ABCB6 contains a unique N-terminal TMD (TMD0), which does not show sequence homology to known proteins. We investigated the functional role of these domains through the molecular dissection of ABCB6. We find that the folding, dimerization, membrane insertion and ATP binding/hydrolysis of the core-ABCB6 complex devoid of TMD0 are preserved. However, in contrast with the full-length transporter, the core-ABCB6 construct is retained at the plasma membrane and does not appear in Rab5-positive endosomes. TMD0 is directly targeted to the lysosomes, without passage to the plasma membrane. Collectively, our results reveal that TMD0 represents an independently folding unit, which is dispensable for catalysis, but has a crucial role in the lysosomal targeting of ABCB6.

Original languageEnglish
Pages (from-to)127-139
Number of pages13
JournalBiochemical Journal
Volume467
Issue number1
DOIs
Publication statusPublished - Apr 1 2015

    Fingerprint

Keywords

  • ATP-binding cassette
  • ATP-binding cassette (ABC) transporter
  • Adenosine 5′-triphosphatase (ATPase)
  • Lysosome
  • Subfamily B (ABCB) 6
  • Trafficking
  • Transmembrane domain

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this