A capsaicin-szenzitív érzö rostok szerepe a patkányuterus kontraktilitásának szabályozá sában

Translated title of the contribution: Role of the capsaicin-sensitive sensory nerve fibers in the regulation of rat - (Uterine) contractile activity

Research output: Contribution to journalArticle

Abstract

The possible role of capsaicin-sensitive sensory nerves in the modulation of neurogenic contractions was studied in non-pregnant and term pregnant rat uteri. Neurogenic contractions were elicited by using electric field stimulation (40 V, 1-70 Hz, 0.6 ms) in intact uteri and in uteri previously exposed to capsaicin in vitro. In the capsaicin pretreated preparations obtained both from non-pregnant and term pregnant rats, a dose-dependent increase in the amplitude of uterine contractions was detected. Prior systemic treatment of rats with capsaicin (130 mg/kg, s.c.) abolished the effect of in vitro capsaicin administration on the amplitude of neurogenic contractions. Use of a specific calcitonin gene-related peptide antagonist revealed that depletion of this peptide, which normally elicits uterine smooth muscle relaxation, may be responsible for the increased responsiveness of the uterus to low frequency stimulation. Experiments on localization of calcitonin gene-related peptide in the uterine tissue specimens exposed to capsaicin revealed a dose-dependent depletion of CGRP-immunoreactive nerves innervating blood vessels and the myometrium. These findings indicate that capsaicin-sensitive afferent nerves, by the release of sensory neuropeptides, significantly contribute to the modulation of uterine contractility both in nonpregnant and term pregnant rats. It is suggested that uterine sensory nerve activation may be a part of a trigger mechanism leading to preterm contractions evoked by, e.g. inflammation.

Original languageHungarian
Pages (from-to)183-190
Number of pages8
JournalMagyar Noorvosok Lapja
Volume67
Issue number4
Publication statusPublished - 2004

Fingerprint

Capsaicin
Nerve Fibers
Uterus
Calcitonin Gene-Related Peptide
Myometrium
Uterine Contraction
Muscle Relaxation
Neuropeptides
Electric Stimulation
Smooth Muscle
Blood Vessels
Inflammation
Peptides

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

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title = "A capsaicin-szenzit{\'i}v {\'e}rz{\"o} rostok szerepe a patk{\'a}nyuterus kontraktilit{\'a}s{\'a}nak szab{\'a}lyoz{\'a} s{\'a}ban",
abstract = "The possible role of capsaicin-sensitive sensory nerves in the modulation of neurogenic contractions was studied in non-pregnant and term pregnant rat uteri. Neurogenic contractions were elicited by using electric field stimulation (40 V, 1-70 Hz, 0.6 ms) in intact uteri and in uteri previously exposed to capsaicin in vitro. In the capsaicin pretreated preparations obtained both from non-pregnant and term pregnant rats, a dose-dependent increase in the amplitude of uterine contractions was detected. Prior systemic treatment of rats with capsaicin (130 mg/kg, s.c.) abolished the effect of in vitro capsaicin administration on the amplitude of neurogenic contractions. Use of a specific calcitonin gene-related peptide antagonist revealed that depletion of this peptide, which normally elicits uterine smooth muscle relaxation, may be responsible for the increased responsiveness of the uterus to low frequency stimulation. Experiments on localization of calcitonin gene-related peptide in the uterine tissue specimens exposed to capsaicin revealed a dose-dependent depletion of CGRP-immunoreactive nerves innervating blood vessels and the myometrium. These findings indicate that capsaicin-sensitive afferent nerves, by the release of sensory neuropeptides, significantly contribute to the modulation of uterine contractility both in nonpregnant and term pregnant rats. It is suggested that uterine sensory nerve activation may be a part of a trigger mechanism leading to preterm contractions evoked by, e.g. inflammation.",
keywords = "Capsaicin-sensitive afferent nerves, Contractility, Pregnancy, Sensory neuropeptides, Uterus",
author = "Anna Klukovits and R. G{\'a}sp{\'a}r and P. S{\'a}ntha and G. Jancs{\'o} and G. Falkay",
year = "2004",
language = "Hungarian",
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T1 - A capsaicin-szenzitív érzö rostok szerepe a patkányuterus kontraktilitásának szabályozá sában

AU - Klukovits, Anna

AU - Gáspár, R.

AU - Sántha, P.

AU - Jancsó, G.

AU - Falkay, G.

PY - 2004

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N2 - The possible role of capsaicin-sensitive sensory nerves in the modulation of neurogenic contractions was studied in non-pregnant and term pregnant rat uteri. Neurogenic contractions were elicited by using electric field stimulation (40 V, 1-70 Hz, 0.6 ms) in intact uteri and in uteri previously exposed to capsaicin in vitro. In the capsaicin pretreated preparations obtained both from non-pregnant and term pregnant rats, a dose-dependent increase in the amplitude of uterine contractions was detected. Prior systemic treatment of rats with capsaicin (130 mg/kg, s.c.) abolished the effect of in vitro capsaicin administration on the amplitude of neurogenic contractions. Use of a specific calcitonin gene-related peptide antagonist revealed that depletion of this peptide, which normally elicits uterine smooth muscle relaxation, may be responsible for the increased responsiveness of the uterus to low frequency stimulation. Experiments on localization of calcitonin gene-related peptide in the uterine tissue specimens exposed to capsaicin revealed a dose-dependent depletion of CGRP-immunoreactive nerves innervating blood vessels and the myometrium. These findings indicate that capsaicin-sensitive afferent nerves, by the release of sensory neuropeptides, significantly contribute to the modulation of uterine contractility both in nonpregnant and term pregnant rats. It is suggested that uterine sensory nerve activation may be a part of a trigger mechanism leading to preterm contractions evoked by, e.g. inflammation.

AB - The possible role of capsaicin-sensitive sensory nerves in the modulation of neurogenic contractions was studied in non-pregnant and term pregnant rat uteri. Neurogenic contractions were elicited by using electric field stimulation (40 V, 1-70 Hz, 0.6 ms) in intact uteri and in uteri previously exposed to capsaicin in vitro. In the capsaicin pretreated preparations obtained both from non-pregnant and term pregnant rats, a dose-dependent increase in the amplitude of uterine contractions was detected. Prior systemic treatment of rats with capsaicin (130 mg/kg, s.c.) abolished the effect of in vitro capsaicin administration on the amplitude of neurogenic contractions. Use of a specific calcitonin gene-related peptide antagonist revealed that depletion of this peptide, which normally elicits uterine smooth muscle relaxation, may be responsible for the increased responsiveness of the uterus to low frequency stimulation. Experiments on localization of calcitonin gene-related peptide in the uterine tissue specimens exposed to capsaicin revealed a dose-dependent depletion of CGRP-immunoreactive nerves innervating blood vessels and the myometrium. These findings indicate that capsaicin-sensitive afferent nerves, by the release of sensory neuropeptides, significantly contribute to the modulation of uterine contractility both in nonpregnant and term pregnant rats. It is suggested that uterine sensory nerve activation may be a part of a trigger mechanism leading to preterm contractions evoked by, e.g. inflammation.

KW - Capsaicin-sensitive afferent nerves

KW - Contractility

KW - Pregnancy

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