Role of the blood-brain barrier in differential response to opioid peptides and morphine in mouse lines divergently bred for high and low swim stress-induced analgesia

Anna Kosson, Istvan Krizbai, Anna Leśniak, Malgorzata Beresewicz, Mariusz Sacharczuk, Piotr Kosson, Peter Nagyoszi, Imola Wilhelm, Patrycja Kleczkowska, Andrzej W. Lipkowski

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Over 20 years ago, the Sadowski group separated two mouse lines, one with high (HA) and the other with low (LA) sensitivity to swim stress-induced analgesia (SSIA). Recently, we proposed that increased leakage of the blood-brain barrier (BBB) in the HA line created the difference in the response to SSIA. To search for further evidence for this hypothesis, differences in the levels of the BBB proteins occludin and claudin-5 were analysed. In addition, we sought to evaluate practical differences in BBB permeability by examining the antinociceptive levels in HA and LA mouse lines after i.v. administration of peptides that have limited access to the CNS. Western blot was used to analyse the differences between occludin and claudin-5. To evaluate the functional differences between the BBB of HA and LA mice, the antinociception levels of endomorphin I, biphalin and AA2016 (peptides with limited BBB permeabilities) in the tail flick test were examined. The expression levels of occludin and claudin-5 in the HA mouse line were lower than in the LA and control mice. Central antinociception of the opioid peptides were significantly higher in the HA line than in the LA and control lines. Our data support the hypothesis that BBB leakage is responsible for the differences between the HA and LA mouse lines. Although SSIA confirmed BBB differences between both lines, it is not limited to the opioid system and could be a useful model for studying the role of the BBB in molecular communications between the periphery and CNS.

Original languageEnglish
Pages (from-to)26-32
Number of pages7
JournalActa neurobiologiae experimentalis
Volume74
Issue number1
Publication statusPublished - Apr 14 2014

Keywords

  • Blood-brain barrier
  • High analgesia mouse line
  • Intrathecal
  • Intravenous
  • Low analgesia mouse line
  • Swim stress-induced analgesia
  • Tail-flick pain test

ASJC Scopus subject areas

  • Neuroscience(all)

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