Role of the Bacterial BacA ABC-Transporter in Chronic Infection of Nodule Cells by Rhizobium Bacteria

Ibtissem Guefrachi, Camille Verly, Éva Kondorosi, Benoît Alunni, Peter Mergaert

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The ability of legumes to acquire sufficient nitrogen from the symbiosis with Rhizobium relies on the intimate contact between the endosymbiotic, intracellular rhizobia, called bacteroids, and their host cells, the symbiotic nodule cells. Nodules contain several thousand symbiotic cells, each harboring thousands of bacteroids. Bacteroids are differentiated bacteria that have a specific metabolism, and in many legumes their formation is accompanied with a morphological transformation involving strong cell enlargement and genome amplification. We have shown that in Medicago and related legumes, the symbiotic nodule cells produce large amounts of highly diverse nodule-specific effector peptides called NCR peptides. These control the bacteroid differentiation by inducing their elongation and DNA amplification. The NCR peptides are similar to antimicrobial peptides (AMPs) of innate immunity in animals and plants and can kill rhizobia in vitro. The BacA protein of Sinorhizobium meliloti provides protection against the antimicrobial activity of NCR peptides and other AMPs with similar mode of action, and intracellular rhizobia require the BacA protein to survive the challenge of the NCR peptides in the nodule cells. BacA is conserved among many bacteria, and several animal pathogens also require BacA or BacA-like proteins for pathogenesis and chronic infection of their host, most likely by providing protection against host-produced innate immunity AMPs. BacA and BacA-like proteins belong to the ABC transporter family. However, what is transported and how this protein provides protection against AMPs remain unknown. Several hypotheses are discussed.

Original languageEnglish
Title of host publicationBiological Nitrogen Fixation
PublisherWiley Blackwell
Pages315-324
Number of pages10
Volume1-2
ISBN (Electronic)9781119053095
ISBN (Print)9781118637043
DOIs
Publication statusPublished - Jan 1 2015

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Keywords

  • Antimicrobial peptide
  • Bacteroid
  • Rhizobium-legume symbiosis

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

Guefrachi, I., Verly, C., Kondorosi, É., Alunni, B., & Mergaert, P. (2015). Role of the Bacterial BacA ABC-Transporter in Chronic Infection of Nodule Cells by Rhizobium Bacteria. In Biological Nitrogen Fixation (Vol. 1-2, pp. 315-324). Wiley Blackwell. https://doi.org/10.1002/9781119053095.ch31