A syndecan-1 szerepe lymphoid sejtek homotípiás aggregációjában

Translated title of the contribution: Role of syndecan-1 in the homotypic aggregation of lymphoid cells

A. Sebestyén, Tótth Árpád, L. Kópper

Research output: Contribution to journalArticle

Abstract

The functions of lymphoid cells are governed by regulatory processes based on the interactions of cells with their microenvironment. More and more proteoglycans are identified in these interactions. We studied the role of syndecan-1, a transmembrane heparan sulfate proteoglycan, in the homotypic adhesions of syndecan- 1 expressing HT58 human NHL cells. Immunohistochemical reactions were performed on HT58 and BL41/95 N1I1. cell lines using confocal laser scanning microscope and flow cytometry. It was shown that homotypic adhesions were inhibited by heparin, heparitinase, anti-heparan sulfate and anti-human syndecan-1 antibodies. When heparitinase was combined with Na-chlorate the inhibition of aggregation was accompanied by an arrest in cell cycle and an increase in apoptotic cells. These experiments proved that the appearance of syndecan-1 on the surface of'HT58 cells contribute to the homotypic adhesion. Moreover, the results also suggest that the heparan sulfate chains, including those on syndecan-1, can influence cell proliferation and active cell death.

Original languageHungarian
Pages (from-to)111-117
Number of pages7
JournalMagyar Onkologia
Volume43
Issue number2
Publication statusPublished - 1999

Fingerprint

Syndecan-1
heparitinsulfate lyase
Lymphocytes
Heparitin Sulfate
Laser Scanning Cytometry
Chlorates
Heparan Sulfate Proteoglycans
Proteoglycans
Cell Cycle Checkpoints
Cell Communication
Heparin
Flow Cytometry
Cell Death
Cell Proliferation
Cell Line
Antibodies

ASJC Scopus subject areas

  • Oncology

Cite this

A syndecan-1 szerepe lymphoid sejtek homotípiás aggregációjában. / Sebestyén, A.; Árpád, Tótth; Kópper, L.

In: Magyar Onkologia, Vol. 43, No. 2, 1999, p. 111-117.

Research output: Contribution to journalArticle

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