The functions of lymphoid cells are governed by regulatory processes based on the interactions of cells with their microenvironment. More and more proteoglycans are identified in these interactions. We studied the role of syndecan-1, a transmembrane heparan sulfate proteoglycan, in the homotypic adhesions of syndecan- 1 expressing HT58 human NHL cells. Immunohistochemical reactions were performed on HT58 and BL41/95 N1I1. cell lines using confocal laser scanning microscope and flow cytometry. It was shown that homotypic adhesions were inhibited by heparin, heparitinase, anti-heparan sulfate and anti-human syndecan-1 antibodies. When heparitinase was combined with Na-chlorate the inhibition of aggregation was accompanied by an arrest in cell cycle and an increase in apoptotic cells. These experiments proved that the appearance of syndecan-1 on the surface of'HT58 cells contribute to the homotypic adhesion. Moreover, the results also suggest that the heparan sulfate chains, including those on syndecan-1, can influence cell proliferation and active cell death.
|Number of pages||7|
|Publication status||Published - Dec 1 1999|
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