Role of sinusoidal heparan sulfate proteoglycan in liver metastasis formation

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Abstract

Previous studies have indicated that the predominant sites of tumor cell extravasation in the liver are the sinusoidal vessels, where tumor cells contact the sinusoidal endothelium and the subendothelial extracellular matrix containing the basic components of the basement membrane. We studied the role of sinusoidal extracellular matrix in metastatsis formation by 3LL-HH murine tumor cells selected for their preferential liver colonization. 3LL-HH tumor cells did not efficiently adhere to cryosections of the liver, but they recognized the sinusoids and vessel walls. Pre-treatment of the mice with polyclonal anti-basement membrane antibodies [anti-laminin, anti-fibronectin and anti-heparan sulfate proteoglycan (HSPG)] significantly modulated the organ distribution of tumor cell colonies following intracardial injection: all 3 antibodies inhibited kidney colonization; anti-laminin and anti-fibronectin antibodies inhibited lung colonization; and only anti-HSPG antibody inhibited liver colonization. In several organs such as the heart, stomach, pancreas and bladder, anti-basement membrane antibody treatment did not alter the process of colonization. Immunofluorescence studies showed that anti-HSPG antibody recognized the basement membranes of sinusoids and blood vessels. Our data suggest a specific involvement of sinusoidal HSPG in the liver colonization of 3LL-HH cells.

Original languageEnglish
Pages (from-to)825-831
Number of pages7
JournalInternational Journal of Cancer
Volume71
Issue number5
DOIs
Publication statusPublished - 1997

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Heparan Sulfate Proteoglycans
Neoplasm Metastasis
Basement Membrane
Liver
Antibodies
Laminin
Neoplasms
Fibronectins
Extracellular Matrix
Anti-Idiotypic Antibodies
Endothelium
Fluorescent Antibody Technique
Blood Vessels
Pancreas
Stomach
Urinary Bladder
Kidney
Lung
Injections
Therapeutics

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

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title = "Role of sinusoidal heparan sulfate proteoglycan in liver metastasis formation",
abstract = "Previous studies have indicated that the predominant sites of tumor cell extravasation in the liver are the sinusoidal vessels, where tumor cells contact the sinusoidal endothelium and the subendothelial extracellular matrix containing the basic components of the basement membrane. We studied the role of sinusoidal extracellular matrix in metastatsis formation by 3LL-HH murine tumor cells selected for their preferential liver colonization. 3LL-HH tumor cells did not efficiently adhere to cryosections of the liver, but they recognized the sinusoids and vessel walls. Pre-treatment of the mice with polyclonal anti-basement membrane antibodies [anti-laminin, anti-fibronectin and anti-heparan sulfate proteoglycan (HSPG)] significantly modulated the organ distribution of tumor cell colonies following intracardial injection: all 3 antibodies inhibited kidney colonization; anti-laminin and anti-fibronectin antibodies inhibited lung colonization; and only anti-HSPG antibody inhibited liver colonization. In several organs such as the heart, stomach, pancreas and bladder, anti-basement membrane antibody treatment did not alter the process of colonization. Immunofluorescence studies showed that anti-HSPG antibody recognized the basement membranes of sinusoids and blood vessels. Our data suggest a specific involvement of sinusoidal HSPG in the liver colonization of 3LL-HH cells.",
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AU - Tóvári, J.

AU - Paku, S.

AU - Rásó, E.

AU - Pogány, Gabor

AU - Kovalszky, I.

AU - Ladányi, A.

AU - Lapis, K.

AU - Tímár, J.

PY - 1997

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