Role of preparative rotation planar chromatography in the isolation of ecdysteroids

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A reliable isolation procedure is elaborated for the purification of ecdysteroids from Serratula wolffii. The procedure is also applicable to other plant sources. The general ecdysteroid isolation procedure was improved by the introduction of preparative rotation planar chromatography (RPC) to the purification process. Effective and simple cleanup and vacuum reversed-phase column chromatographic separation was completed with RPC, or repeated RPC, to obtain pure ecdysteroids (ajugasterone C, dacryhainansterone, 22-deoxy-integristerone A, 20-hydroxyecdysone, and 2 new ecdysteroids) from the crude extract. This paper discusses the advantages of this method as the final step of ecdysteroid isolation.

Original languageEnglish
Pages (from-to)2095-2109
Number of pages15
JournalJournal of Liquid Chromatography and Related Technologies
Issue number14
Publication statusPublished - Aug 1 2006


  • Ecdysteroids
  • Plant material
  • Rotation planar chromatography
  • Serratula wolffii

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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