Role of phospholipids containing docosahexaenoyl chains in modulating the activity of protein kinase C

Jennifer Giorgione, Richard M. Epand, Csaba Buda, T. Farkas

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

It is known that the phospholipids of the brain cells of fish are altered during cold adaptation. In particular, the 1-monounsaturated 2- polyunsaturated phosphatidylethanolamines (PEs) increase 2- to 3-fold upon adaptation to cold. One of the most striking changes is in the 18:1/22:6 species of PE. We determined how this lipid affected the bilayer-to- hexagonal-phase transition temperature of 16:1/16:1 PE. We found that it was more effective in lowering this transition temperature than were other, less unsaturated, PE species. In addition, it was not simply the presence of the 18:1/22:6 acyl chains which caused this effect, since the 18:1/22:6 species of phosphatidylcholine had the opposite effect on this transition temperature. Zwitterionic substances that lower the bilayer-to-hexagonal- phase transition temperature often cause an increase in the activity of protein kinase C (PKC). Indeed, the 18:1/22:6 PE caused an increase in the rate of histone phosphorylation by PKC which was greater than that caused by other, less unsaturated, PEs. The 18:1/22:6 phosphatidylcholine had no effect on this enzyme. The stimulation of the activity of PKC by the 18:1/22:6 PE is a consequence of this lipid's increasing the partitioning of PKC to the membrane.

Original languageEnglish
Pages (from-to)9767-9770
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number21
DOIs
Publication statusPublished - Oct 10 1995

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Phosphatidylethanolamines
Protein Kinase C
Phospholipids
Transition Temperature
Phase Transition
Phosphatidylcholines
Lipid Bilayers
Histones
Fishes
Phosphorylation
Lipids
Membranes
Brain
Enzymes

Keywords

  • docosahexaenoic acid
  • phosphatidylethanolamine
  • thermal acclimatization

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Role of phospholipids containing docosahexaenoyl chains in modulating the activity of protein kinase C. / Giorgione, Jennifer; Epand, Richard M.; Buda, Csaba; Farkas, T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 21, 10.10.1995, p. 9767-9770.

Research output: Contribution to journalArticle

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