Role of nucleotides and phosphoinositides in the stability of electron and proton currents associated with the phagocytic NADPH oxidase

Gábor L. Petheo, Nathalie C. Girardin, Nicolas Goossens, Gergely Z. Molnar, Nicolas Demaurex

Research output: Contribution to journalArticle

6 Citations (Scopus)


The phagocytic NADPH oxidase (phox) moves electrons across cell membranes to kill microbes. The activity of this lethal enzyme is tightly regulated, but the mechanisms that control phox inactivation are poorly understood for lack of appropriate assays. The phox generates measurable electron currents, I e, that are associated with inward proton currents, IH. To study the inactivation of the phox and of its associated proton channel, we determined which soluble factors can stabilize Ie (induced by the addition of NADPH) and IH (initiated by small depolarizing voltage steps) in inside-out patches from PMA-activated human eosinophils. Ie decayed rapidly in the absence of nucleotides (τ ≈ 6 min) and was maximally stabilized by the combined addition of 5 mM ATP and 50 μM of the non-hydrolysable GTP analogue GTP[S] (guanosine 5′-[γ-thio] triphosphate) (τ ≈ 57 min), but not by either ATP or GTP[S] alone. I H also decayed rapidly and was stabilized by the ATP/GTP[S] mixture, but maximal stabilization of IH required further addition of 25 μM PI(3,4)P2 (phosphoinositide 3,4-bisphosphate) to the cytosolic side of the patch. PI(3,4)P2 had no effect on Ie and its stabilizing effect on IH could not be mimicked by other phosphoinositides. Reducing the ATP concentration below millimolar levels decreased IH stability, an effect that was not prevented by phosphatase inhibitors but by the non-hydrolysable ATP analogue ATP[S] (adenosine 5′-[γ-thio]triphosphate). Our data indicate that the assembled phox complex is very stable in eosinophil membranes if both ATP and GTP[S] are present, but inactivates within minutes if one of the nucleotides is removed. Stabilization of the phox-associated proton channel in a highly voltage-sensitive conformation does not appear to involve phosphorylation but ATP binding, and requires not only ATP and GTP[S] but also PI(3,4)P2, a protein known to anchor the cytosolic phox subunit p47phox to the plasma membrane.

Original languageEnglish
Pages (from-to)431-438
Number of pages8
JournalBiochemical Journal
Issue number3
Publication statusPublished - Dec 15 2006


  • Cytochrome
  • Eosinophil
  • NADPH oxidase
  • Patch-clamp
  • Phosphoinositide
  • Proton channel

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Role of nucleotides and phosphoinositides in the stability of electron and proton currents associated with the phagocytic NADPH oxidase'. Together they form a unique fingerprint.

  • Cite this