Role of N - Or C -terminal biotinylation in autoantibody recognition of citrullin containing filaggrin epitope peptides in rheumatoid arthritis

Fruzsina Babos, Eszter Szarka, György Nagy, Zs. Majer, G. Sármay, Anna Magyar, F. Hudecz

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Here, we report on the synthesis, conformational analysis, and autoantibody binding properties of new sets of rheumatoid arthritis (RA) specific biotin-peptide conjugates derived from filaggrin epitope peptides. The biotin with or without a linker was attached to the Cit or Arg containing epitope core (311TXGRS315) or epitope region ( 306SHQESTXGXSXGRSGRSGS324) peptide (where X = Cit), through an amide bond at the N- or C-terminal of the epitopes. Antibody binding was detected by indirect enzyme-linked immunosorbent assay (ELISA) using sera from RA, Systemic lupus erythematosus (SLE) patients, as well as healthy individuals, and the secondary structure of conjugates was investigated by electronic circular dichroism (ECD). We found that autoantibodies from RA patients recognize specifically both filaggrin epitope region ( 306SHQESTXGXSXGRSGRSGS324) and short epitope core ( 311TXGRS315) peptides. Our data also indicate that the positioning of the biotin label within a peptide sequence can markedly influence the antibody binding, but the length of the linker incorporated has essentially no effect on the recognition. ECD experiments demonstrate that the Arg/Cit change does not influence the solution conformation of the peptide conjugates. However, the presence and position of the biotin moiety has a pronounced effect on the conformation of the 5-mer epitope core peptides, while it does not alter the secondary structure of the 19-mer epitope region peptides.

Original languageEnglish
Pages (from-to)817-827
Number of pages11
JournalBioconjugate Chemistry
Volume24
Issue number5
DOIs
Publication statusPublished - May 15 2013

Fingerprint

Biotinylation
Epitopes
Autoantibodies
Peptides
Rheumatoid Arthritis
Biotin
Dichroism
Circular Dichroism
Antibodies
Conformations
Immunosorbents
filaggrin
Amides
Systemic Lupus Erythematosus
Labels
Assays
Enzymes
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Organic Chemistry
  • Pharmaceutical Science
  • Biomedical Engineering
  • Pharmacology

Cite this

Role of N - Or C -terminal biotinylation in autoantibody recognition of citrullin containing filaggrin epitope peptides in rheumatoid arthritis. / Babos, Fruzsina; Szarka, Eszter; Nagy, György; Majer, Zs.; Sármay, G.; Magyar, Anna; Hudecz, F.

In: Bioconjugate Chemistry, Vol. 24, No. 5, 15.05.2013, p. 817-827.

Research output: Contribution to journalArticle

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