Role of lipid polymorphism in G protein-membrane interactions: Nonlamellar-prone phospholipids and peripheral protein binding to membranes

Pablo V. Escribá, Andrés Ozaita, Catalina Ribas, Antonio Miralles, E. Fodor, T. Farkas, Jesús A. García-Sevilla

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Heterotrimeric G proteins (peripheral proteins) conduct signals from membrane receptors (integral proteins) to regulatory proteins localized to various cellular compartments. They are in excess over any G protein-coupled receptor type on the cell membrane, which is necessary for signal amplification. These facts account for the large number of G protein molecules bound to membrane lipids. Thus, the protein-lipid interactions are crucial for their cellular localization, and consequently for signal transduction. In this work, the binding of G protein subunits to model membranes (liposomes), formed with defined membrane lipids, has been studied. It is shown that although G protein α-subunits were able to bind to lipid bilayers, the presence of nonlamellar-prone phospholipids (phosphatidylethanolamines) enhanced their binding to model membranes. This mechanism also appears to be used by other (structurally and functionally unrelated) peripheral proteins, such as protein kinase C and the insect protein apolipophorin III, indicating that it could constitute a general mode of protein-lipid interactions, relevant in the activity and translocation of some peripheral (amphitropic) proteins from soluble to particulate compartments. Other factors, such as the presence of cholesterol or the vesicle surface charge, also modulated the binding of the G protein subunits to lipid bilayers. Conversely, the binding of G protein-coupled receptor kinase 2 and the G protein β-subunit to liposomes was not increased by hexagonally prone lipids. Their distinct interactions with membrane lipids may, in part, explain the different cellular localizations of all of these proteins during the signaling process.

Original languageEnglish
Pages (from-to)11375-11380
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number21
DOIs
Publication statusPublished - Oct 14 1997

Fingerprint

GTP-Binding Proteins
Protein Binding
Phospholipids
Lipids
Protein Subunits
Membranes
Membrane Lipids
Proteins
Carrier Proteins
Lipid Bilayers
Liposomes
G-Protein-Coupled Receptor Kinase 2
Insect Proteins
Phosphatidylethanolamines
Heterotrimeric GTP-Binding Proteins
G-Protein-Coupled Receptors
Protein Kinase C
Signal Transduction
Membrane Proteins
Cholesterol

Keywords

  • Hexagonal phase
  • Phosphatidylethanolamine
  • Protein kinase C
  • Signal transduction

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Role of lipid polymorphism in G protein-membrane interactions : Nonlamellar-prone phospholipids and peripheral protein binding to membranes. / Escribá, Pablo V.; Ozaita, Andrés; Ribas, Catalina; Miralles, Antonio; Fodor, E.; Farkas, T.; García-Sevilla, Jesús A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 21, 14.10.1997, p. 11375-11380.

Research output: Contribution to journalArticle

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