Role of GDF15 in radiosensitivity of breast cancer cells

Boglárka Schilling-Tóth, Nikolett Sándor, Fruzsina R. Walter, Alexandra Bocsik, G. Sáfrány, H. Hegyesi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The Growth Differentiation Factor-15 gene (GDF15) is a member of TGF-b superfamily and this cytokine family is considered to be a promising target for cancer therapy. The purpose of this study was to investigate the effect of tumor derived GDF15 on proliferation and radiosensitivity of breast cancer cells in vitro and in vivo. A mouse breast cancer LM2 cell line with stable transfection of full-length mouse GDF15 cDNA was established. Cell growth and proliferation was observed using WST assay and impedance-based method. Radiation induced GDF15 and TGF-b1 expression was determined by qRT-PCR. Radiosensitivity was measured by a colony formation assay in vitro and by a tumor growth delay assay in vivo. Cells with more than a 10-fold increase in GDF15 expression had a higher growth rate than parental control cells in vitro and in vivo. The radiation induced elevation of the expression of TGFb1 was reduced in GDF15 overexpressing cells. GDF15 may play a role in the radiation response of breast cancer cells by effecting cell survival, inhibiting radiation-induced cell death, and inhibiting the TGF-b1 related cytotoxic action.

Original languageEnglish
Pages (from-to)982-992
Number of pages11
JournalCentral European Journal of Biology
Volume9
Issue number10
DOIs
Publication statusPublished - 2014

Fingerprint

Growth Differentiation Factor 15
growth differentiation factors
Radiation Tolerance
breast neoplasms
Genes
Cells
Breast Neoplasms
Radiation
genes
Assays
neoplasms
Tumors
assays
Growth
Neoplasms
mice
Cell proliferation
impedance
Cell growth
Cell death

Keywords

  • Biomarker
  • Breast cancer
  • GDF15
  • Radiosensitivity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Role of GDF15 in radiosensitivity of breast cancer cells. / Schilling-Tóth, Boglárka; Sándor, Nikolett; Walter, Fruzsina R.; Bocsik, Alexandra; Sáfrány, G.; Hegyesi, H.

In: Central European Journal of Biology, Vol. 9, No. 10, 2014, p. 982-992.

Research output: Contribution to journalArticle

Schilling-Tóth, Boglárka ; Sándor, Nikolett ; Walter, Fruzsina R. ; Bocsik, Alexandra ; Sáfrány, G. ; Hegyesi, H. / Role of GDF15 in radiosensitivity of breast cancer cells. In: Central European Journal of Biology. 2014 ; Vol. 9, No. 10. pp. 982-992.
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