Role of fructose-1,6-bisphosphatase, fructose phosphotransferase, and phosphofructokinase in saccharide metabolism of four C3 grassland species under elevated CO2

E. Nádas, Á Balogh, F. Kiss, K. Szente, Z. Nagy, R. Martínez-Carrasco, Z. Tuba

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We studied the effects of 15-months of elevated (700 μmol mol -1) CO2 concentration (EC) on the CO2 assimilation rate, saccharide content, and the activity of key enzymes in the regulation of saccharide metabolism (glycolysis and gluconeogenesis) of four C3 perennial temperate grassland species, the dicots Filipendula vulgaris and Salvia nemorosa and the monocots Festuca rupicola and Dactylis glomerata. The acclimation of photosynthesis to EC was downward in F. rupicola and D. glomerata whereas it was upward in F. vulgaris and S. nemorosa. At EC, F. rupicola and F. vulgaris leaves accumulated starch while soluble sugar contents were higher in F. vulgaris and D. glomerata. EC decreased pyrophosphate-D- fructose-6-phosphate l-phosphotransferase (PFP, EC 2.7.1.90) activity assayed with Fru-2,6-P2 in F. vulgaris and D. glomerata and increased it in F. rupicola and S. nemorosa. Growth in EC decreased phosphofructokinase (PFK, EC 2.7.1.11) activity in all four species, the decrease being smallest in S. nemorosa and greatest in F. rupicola. With Fru-2,6-P2 in the assay medium, EC increased the PFP/PFK ratio, except in F. vulgaris. Cytosolic fructose-1,6-bisphosphatase (Fru-1,6-P2ase, EC 3.1.3.11) was inhibited by EC, the effect being greatest in F. vulgaris and smallest in F. rupicola. Glucose-6-phosphate dehydrogenase (G6PDH EC 1.1.1.49) activity was decreased by growth EC in the four species. Activity ratios of Fru-1,6-P 2ase to PFP and PFK suggest that EC may shift sugar metabolism towards glycolysis in the dicots.

Original languageEnglish
Pages (from-to)255-261
Number of pages7
JournalPhotosynthetica
Volume46
Issue number2
DOIs
Publication statusPublished - Jun 1 2008

Keywords

  • Acclimation
  • CO assimilation
  • Fructose-1,6-bisphosphatase
  • Gluconeogenesis
  • Glucose-6-phosphate dehydrogenase
  • Glycolysis
  • Oxidative pentose phosphate pathway
  • Phosphofructokinase
  • Pyrophosphate D-fructose-6-phosphate l-phosphotransferase
  • Starch

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Fingerprint Dive into the research topics of 'Role of fructose-1,6-bisphosphatase, fructose phosphotransferase, and phosphofructokinase in saccharide metabolism of four C<sub>3</sub> grassland species under elevated CO<sub>2</sub>'. Together they form a unique fingerprint.

  • Cite this