Role of extracellular polysaccharide (EPS) slime of plant pathogenic bacteria in protecting cells to reactive oxygen species

Z. Király, H. M. El-Zahaby, Z. Klement

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Pseudomonas syringae pv. phaseolicola, a phytopathogenic bacterium, seemed very sensitive in planta to the adverse action of reactive oxygen species (ROS) produced by two chemical systems. The disease symptoms in host plants were also suppressed by ROS. Several other plant pathogenic bacteria (P. syringae pv. pisi, Erwinia arnylovora, Xanthomonas campestris pv. pelargonii) as well as P. fluorescens were also sensitive in vitro to the inhibiting or killing action of ROS. It was shown that O2.- and H2O2, were produced in our two chemical systems and were involved in the killing action. OH. however was not involved in the adverse action on bacteria of the ROS. Superoxide dismutase and catalase were able to reverse the killing action of ROS. When the EPS slime around bacteria was removed by washing and centrifuging the cells, bacteria were more sensitive to ROS. However, when the cells of EPS- mutants were washed and centrifuged, their sensitivity to the killing action of ROS did not change because the lack of slime around the mutant cells. The EPS- Tn5 mutants of P. syringae pv. phaseolicola and the natural EPS- mutant of E. amylovora were more sensitive to ROS than the wild type strains. These results support the idea that the EPS slime protects bacteria from ROS (O2.- and H2O2).

Original languageEnglish
Pages (from-to)59-68
Number of pages10
JournalJournal of Phytopathology
Volume145
Issue number2-3
DOIs
Publication statusPublished - Jan 1 1997

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ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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