Role of environmental temperature in seasonal changes of fatty acid composition of hepatic lipid in an air-breathing Indian teleost, Channa punctatus (Bloch)

Haridas Dutta, Anuradha Das, Asit Baran Das, T. Farkas

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

1. 1. Fatty acid composition of hepatic total lipids and phospholipids of an indian air-breathing fish, Channa punctatus Bloch, collected in summer (32°C) or in winter (16°C) or after exposing summer and winter adapted fish to the opposite temperatures for one month was investigated. DPH fluorescence polarisation of phospholipid vesicles was also monitored. 2. 2. There was a tendency to accumulate eicosa- and docosapolyenoic fatty acids to the expense of monoenoic acids in the winter fish livers. This tendency was not so clear in the case of isolated phospholipids. However, summer adapted fish acclimated to 16°C contained significantly more docosapolyenoic acids in their phospholipids than winter adapted fish acclimated to 32°C. 3. 3. Phospholipid vesicles obtained from summer adapted fish livers proved to be more rigid than those from winter adapted ones. Fluorescence polarisation vs I/T plots revealed two breaks indicating phase separations at 25 and 15°C, respectively. No such breaks were evident with phospholipids obtained from winter adapted fish. 4. 4. When winter adapted fish were acclimated to 32°C the fluidity of vesicles, including the appearance of phase separation temperatures, become very similar to the summer adapted fish suggesting a complete adaptation of membrane physical state to temperature. Exposing summer adapted fish to 16°C resulted in the disappearance of phase separation temperatures observed at 32°C but these vesicles still exhibited higher polarisation values. 5. 5. It is suggested that for the complete adaptation of membrane physical state to temperature in warm adapted 16°C acclimated fish, some other factors than phospholipid fatty acid composition are required.

Original languageEnglish
Pages (from-to)341-347
Number of pages7
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume81
Issue number2
DOIs
Publication statusPublished - 1985

Fingerprint

Fish
Respiration
Fishes
Fatty Acids
Air
Lipids
Temperature
Liver
Phospholipids
Chemical analysis
Phase separation
Fluorescence Polarization
Polarization
Fluorescence
Membranes
Acids
Fluidity

Cite this

@article{e44632ccfb2a405cb8d5880a8a773013,
title = "Role of environmental temperature in seasonal changes of fatty acid composition of hepatic lipid in an air-breathing Indian teleost, Channa punctatus (Bloch)",
abstract = "1. 1. Fatty acid composition of hepatic total lipids and phospholipids of an indian air-breathing fish, Channa punctatus Bloch, collected in summer (32°C) or in winter (16°C) or after exposing summer and winter adapted fish to the opposite temperatures for one month was investigated. DPH fluorescence polarisation of phospholipid vesicles was also monitored. 2. 2. There was a tendency to accumulate eicosa- and docosapolyenoic fatty acids to the expense of monoenoic acids in the winter fish livers. This tendency was not so clear in the case of isolated phospholipids. However, summer adapted fish acclimated to 16°C contained significantly more docosapolyenoic acids in their phospholipids than winter adapted fish acclimated to 32°C. 3. 3. Phospholipid vesicles obtained from summer adapted fish livers proved to be more rigid than those from winter adapted ones. Fluorescence polarisation vs I/T plots revealed two breaks indicating phase separations at 25 and 15°C, respectively. No such breaks were evident with phospholipids obtained from winter adapted fish. 4. 4. When winter adapted fish were acclimated to 32°C the fluidity of vesicles, including the appearance of phase separation temperatures, become very similar to the summer adapted fish suggesting a complete adaptation of membrane physical state to temperature. Exposing summer adapted fish to 16°C resulted in the disappearance of phase separation temperatures observed at 32°C but these vesicles still exhibited higher polarisation values. 5. 5. It is suggested that for the complete adaptation of membrane physical state to temperature in warm adapted 16°C acclimated fish, some other factors than phospholipid fatty acid composition are required.",
author = "Haridas Dutta and Anuradha Das and Das, {Asit Baran} and T. Farkas",
year = "1985",
doi = "10.1016/0305-0491(85)90323-2",
language = "English",
volume = "81",
pages = "341--347",
journal = "Comparative biochemistry and physiology. B, Comparative biochemistry",
issn = "1096-4959",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Role of environmental temperature in seasonal changes of fatty acid composition of hepatic lipid in an air-breathing Indian teleost, Channa punctatus (Bloch)

AU - Dutta, Haridas

AU - Das, Anuradha

AU - Das, Asit Baran

AU - Farkas, T.

PY - 1985

Y1 - 1985

N2 - 1. 1. Fatty acid composition of hepatic total lipids and phospholipids of an indian air-breathing fish, Channa punctatus Bloch, collected in summer (32°C) or in winter (16°C) or after exposing summer and winter adapted fish to the opposite temperatures for one month was investigated. DPH fluorescence polarisation of phospholipid vesicles was also monitored. 2. 2. There was a tendency to accumulate eicosa- and docosapolyenoic fatty acids to the expense of monoenoic acids in the winter fish livers. This tendency was not so clear in the case of isolated phospholipids. However, summer adapted fish acclimated to 16°C contained significantly more docosapolyenoic acids in their phospholipids than winter adapted fish acclimated to 32°C. 3. 3. Phospholipid vesicles obtained from summer adapted fish livers proved to be more rigid than those from winter adapted ones. Fluorescence polarisation vs I/T plots revealed two breaks indicating phase separations at 25 and 15°C, respectively. No such breaks were evident with phospholipids obtained from winter adapted fish. 4. 4. When winter adapted fish were acclimated to 32°C the fluidity of vesicles, including the appearance of phase separation temperatures, become very similar to the summer adapted fish suggesting a complete adaptation of membrane physical state to temperature. Exposing summer adapted fish to 16°C resulted in the disappearance of phase separation temperatures observed at 32°C but these vesicles still exhibited higher polarisation values. 5. 5. It is suggested that for the complete adaptation of membrane physical state to temperature in warm adapted 16°C acclimated fish, some other factors than phospholipid fatty acid composition are required.

AB - 1. 1. Fatty acid composition of hepatic total lipids and phospholipids of an indian air-breathing fish, Channa punctatus Bloch, collected in summer (32°C) or in winter (16°C) or after exposing summer and winter adapted fish to the opposite temperatures for one month was investigated. DPH fluorescence polarisation of phospholipid vesicles was also monitored. 2. 2. There was a tendency to accumulate eicosa- and docosapolyenoic fatty acids to the expense of monoenoic acids in the winter fish livers. This tendency was not so clear in the case of isolated phospholipids. However, summer adapted fish acclimated to 16°C contained significantly more docosapolyenoic acids in their phospholipids than winter adapted fish acclimated to 32°C. 3. 3. Phospholipid vesicles obtained from summer adapted fish livers proved to be more rigid than those from winter adapted ones. Fluorescence polarisation vs I/T plots revealed two breaks indicating phase separations at 25 and 15°C, respectively. No such breaks were evident with phospholipids obtained from winter adapted fish. 4. 4. When winter adapted fish were acclimated to 32°C the fluidity of vesicles, including the appearance of phase separation temperatures, become very similar to the summer adapted fish suggesting a complete adaptation of membrane physical state to temperature. Exposing summer adapted fish to 16°C resulted in the disappearance of phase separation temperatures observed at 32°C but these vesicles still exhibited higher polarisation values. 5. 5. It is suggested that for the complete adaptation of membrane physical state to temperature in warm adapted 16°C acclimated fish, some other factors than phospholipid fatty acid composition are required.

UR - http://www.scopus.com/inward/record.url?scp=0008120689&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0008120689&partnerID=8YFLogxK

U2 - 10.1016/0305-0491(85)90323-2

DO - 10.1016/0305-0491(85)90323-2

M3 - Article

AN - SCOPUS:0008120689

VL - 81

SP - 341

EP - 347

JO - Comparative biochemistry and physiology. B, Comparative biochemistry

JF - Comparative biochemistry and physiology. B, Comparative biochemistry

SN - 1096-4959

IS - 2

ER -