Role of domain interactions in the collective motion of phosphoglycerate kinase

Gusztáv Schay, Levente Herényi, J. Fidy, Szabolcs Osváth

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Protein function is governed by the underlying conformational dynamics of the molecule. The experimental and theoretical work leading to contemporary understanding of enzyme dynamics was mostly restricted to the large-scale movements of single-domain proteins. Collective movements resulting from a regulatory interplay between protein domains is often crucial for enzymatic activity. It is not clear, however, how our knowledge could be extended to describe collective near-equilibrium motions of multidomain enzymes. We examined the effect of domain interactions on the low temperature near equilibrium dynamics of the native state, using phosphoglycerate kinase as model protein. We measured thermal activation of tryptophan phosphorescence quenching to explore millisecond-range protein motions. The two protein domains of phosphoglycerate kinase correspond to two dynamic units, but interdomain interactions link the motion of the two domains. The effect of the interdomain interactions on the activation of motions in the individual domains is asymmetric. As the temperature of the frozen protein is increased from the cryogenic, motions of the N domain are activated first. This is a partial activation, however, and the full dynamics of the domain becomes activated only after the activation of the C domain.

Original languageEnglish
Pages (from-to)677-682
Number of pages6
JournalBiophysical Journal
Volume104
Issue number3
DOIs
Publication statusPublished - Feb 5 2013

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Phosphoglycerate Kinase
Proteins
Temperature
Enzymes
Articular Range of Motion
Tryptophan
Hot Temperature
Protein Domains

ASJC Scopus subject areas

  • Biophysics

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Role of domain interactions in the collective motion of phosphoglycerate kinase. / Schay, Gusztáv; Herényi, Levente; Fidy, J.; Osváth, Szabolcs.

In: Biophysical Journal, Vol. 104, No. 3, 05.02.2013, p. 677-682.

Research output: Contribution to journalArticle

Schay, Gusztáv ; Herényi, Levente ; Fidy, J. ; Osváth, Szabolcs. / Role of domain interactions in the collective motion of phosphoglycerate kinase. In: Biophysical Journal. 2013 ; Vol. 104, No. 3. pp. 677-682.
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