Role of direct bioautographic method for detection of antistaphylococcal activity of essential oils

G. Horváth, Noémi Jámbor, Erika Kocsis, Andrea Böszörményi, E. Lemberkovics, Éva Héthelyi, Krisztina Kovács, Béla Kocsis

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The aim of the present study was the chemical characterization of some traditionally used and therapeutically relevant essential oils (thyme, eucalyptus, cinnamon bark, clove, and tea tree) and the optimized microbiological investigation of the effect of these oils on clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin- susceptible S. aureus (MSSA). The chemical composition of the oils was analyzed by TLC, and controlled by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The antibacterial effect was investigated using a TLC-bioautographic method. Antibacterial activity of thyme, clove and cinnamon oils, as well as their main components (thymol, carvacrol, eugenol, and cinnamic aldehyde) was observed against all the bacterial strains used in this study. The essential oils of eucalyptus and tea tree showed weak activity in the bioautographic system. On the whole, the antibacterial activity of the essential oils could be related to their most abundant components, but the effect of the minor components should also be taken into consideration. Direct bioautography is more cost-effective and better in comparison with traditional microbiological laboratory methods (e.g. disc-diffusion, agar-plate technique).

Original languageEnglish
Pages (from-to)1379-1384
Number of pages6
JournalNatural Product Communications
Volume6
Issue number9
Publication statusPublished - 2011

Fingerprint

Volatile Oils
Thymus Plant
Cinnamomum zeylanicum
Eucalyptus
essential oils
thyme
Tea
tea
Oils
Clove Oil
cinnamon oil
Thymol
Syzygium
Oil and Gas Fields
clove oil
Eugenol
oils
Methicillin
cinnamon
cloves

Keywords

  • Antibacterial activity
  • Direct bioautography
  • Essential oils
  • Methicillin-resistant Staphylococcus aureus (MRSA)
  • Thin layer chromatography

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Complementary and alternative medicine
  • Plant Science

Cite this

Horváth, G., Jámbor, N., Kocsis, E., Böszörményi, A., Lemberkovics, E., Héthelyi, É., ... Kocsis, B. (2011). Role of direct bioautographic method for detection of antistaphylococcal activity of essential oils. Natural Product Communications, 6(9), 1379-1384.

Role of direct bioautographic method for detection of antistaphylococcal activity of essential oils. / Horváth, G.; Jámbor, Noémi; Kocsis, Erika; Böszörményi, Andrea; Lemberkovics, E.; Héthelyi, Éva; Kovács, Krisztina; Kocsis, Béla.

In: Natural Product Communications, Vol. 6, No. 9, 2011, p. 1379-1384.

Research output: Contribution to journalArticle

Horváth, G, Jámbor, N, Kocsis, E, Böszörményi, A, Lemberkovics, E, Héthelyi, É, Kovács, K & Kocsis, B 2011, 'Role of direct bioautographic method for detection of antistaphylococcal activity of essential oils', Natural Product Communications, vol. 6, no. 9, pp. 1379-1384.
Horváth, G. ; Jámbor, Noémi ; Kocsis, Erika ; Böszörményi, Andrea ; Lemberkovics, E. ; Héthelyi, Éva ; Kovács, Krisztina ; Kocsis, Béla. / Role of direct bioautographic method for detection of antistaphylococcal activity of essential oils. In: Natural Product Communications. 2011 ; Vol. 6, No. 9. pp. 1379-1384.
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