Role of conserved non-coding regulatory elements in lMW glutenin gene expression

Angéla Juhász, Szabolcs Makai, Endre Sebestyén, L. Tamás, E. Balázs

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Transcriptional regulation of LMW glutenin genes were investigated in-silico, using publicly available gene sequences and expression data. Genes were grouped into different LMW glutenin types and their promoter profiles were determined using cis-acting regulatory elements databases and published results. The various cis-acting elements belong to some conserved non-coding regulatory regions (CREs) and might act in two different ways. There are elements, such as GCN4 motifs found in the long endosperm box that could serve as key factors in tissue-specific expression. Some other elements, such as the AACA/TA motifs or the individual prolamin box variants, might modulate the level of expression. Based on the promoter sequences and expression characteristic LMW glutenin genes might be transcribed following two different mechanisms. Most of the s- and i-type genes show a continuously increasing expression pattern. The m-type genes, however, demonstrate normal distribution in their expression profiles. Differences observed in their expression could be related to the differences found in their promoter sequences. Polymorphisms in the number and combination of cis-acting elements in their promoter regions can be of crucial importance in the diverse levels of production of single LMW glutenin gene types.

Original languageEnglish
Article numbere29501
JournalPLoS One
Volume6
Issue number12
DOIs
Publication statusPublished - Dec 29 2011

Fingerprint

glutenins
Gene expression
Genes
Gene Expression
gene expression
promoter regions
genes
Prolamins
Endosperm
regulatory sequences
prolamins
Nucleic Acid Regulatory Sequences
Normal Distribution
Thromboplastin
Genetic Promoter Regions
Computer Simulation
Normal distribution
endosperm
glutenin
Polymorphism

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Role of conserved non-coding regulatory elements in lMW glutenin gene expression. / Juhász, Angéla; Makai, Szabolcs; Sebestyén, Endre; Tamás, L.; Balázs, E.

In: PLoS One, Vol. 6, No. 12, e29501, 29.12.2011.

Research output: Contribution to journalArticle

Juhász, Angéla ; Makai, Szabolcs ; Sebestyén, Endre ; Tamás, L. ; Balázs, E. / Role of conserved non-coding regulatory elements in lMW glutenin gene expression. In: PLoS One. 2011 ; Vol. 6, No. 12.
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