Role of chemical structure in molecular recognition by transferrin

Anikó Takátsy, Judit Hodrea, Cornelia Majdik, Florin Dan Irimie, F. Kilár

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Studies of molecular recognition of chiral compounds by proteins are of importance from many points of view. The biological role of proteins in their interaction with small molecules is of fundamental interest and can be used in many different fields, for instance for in vitro analysis of optically active compounds. Studies in these areas need a detailed study of the interaction sites on the protein surface and the relationship between chemical structure and the complex formation ability of small molecules, such as drugs. The electrophoretic migration of charged compounds through a protein zone may provide information about the surface properties of the macromolecule in the interaction site. The interaction of human serum transferrin with tryptophan-methyl- (TME), ethyl- (TEE) and butyl-esters (TBE) has been investigated by capillary electrophoresis (CE) and model calculations. Differences in the separation of tryptophan derivatives were obtained by varying experimental parameters such as, pH, ionic strength of background electrolyte and the length of transferrin zone. Limited separation of the enantiomer pairs were observed at pH 5 and 7 with a maximum resolution at pH 6. The size of the ligands coupled to the chiral centre has importance in stereoselective recognition; however, a direct comparison of resolution different in same runs may lead to false conclusion if the experimental conditions are not comparable. With a careful evaluation of the data we obtained significant differences between the resolution of the smallest enantiomer pair compared to those of tryptophan derivatives with longer alkyl chains.

Original languageEnglish
Pages (from-to)270-274
Number of pages5
JournalJournal of Molecular Recognition
Volume19
Issue number4
DOIs
Publication statusPublished - Jul 2006

Fingerprint

Molecular recognition
Transferrin
Molecular Structure
Enantiomers
Proteins
Tryptophan
Derivatives
Capillary electrophoresis
Molecules
Surface Properties
Capillary Electrophoresis
Ionic strength
Macromolecules
Osmolar Concentration
Electrolytes
Surface properties
Membrane Proteins
Esters
Ligands
Serum

Keywords

  • Capillary electrophoresis
  • Chemical structure
  • Chiral separation
  • Human serum transferrin
  • Molecular recognition
  • pH dependence

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Computer Vision and Pattern Recognition
  • Immunology
  • Molecular Biology

Cite this

Role of chemical structure in molecular recognition by transferrin. / Takátsy, Anikó; Hodrea, Judit; Majdik, Cornelia; Irimie, Florin Dan; Kilár, F.

In: Journal of Molecular Recognition, Vol. 19, No. 4, 07.2006, p. 270-274.

Research output: Contribution to journalArticle

Takátsy, Anikó ; Hodrea, Judit ; Majdik, Cornelia ; Irimie, Florin Dan ; Kilár, F. / Role of chemical structure in molecular recognition by transferrin. In: Journal of Molecular Recognition. 2006 ; Vol. 19, No. 4. pp. 270-274.
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