Role of bradykinin in the hyperaemia following acid challenge of the rat gastric mucosa

Gábor Pethö, Milana Jocič, Peter Holzer

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This study examined whether the hyperaemia following acid challenge of the rat gastric mucosa involves bradykinin, a peptide formed in response to tissue injury. Gastric mucosal blood flow in urethane‐anaesthetized rats was assessed by the hydrogen gas clearance method. Infusion of a bradykinin solution (10 μm) into the gastric wall augmented gastric mucosal blood flow by a factor of 2.3, an effect that was prevented by the bradykinin B2 antagonist Hoe‐140 (icatibant; 100 μmol kg−1, i.v.). I.v. injection of bradykinin (20–60 nmol kg−1) caused a 2.3‐3.5 fold increase in blood flow through the left gastric artery as measured by the ultrasonic transit time shift technique. The hyperaemic effect of bradykinin in this gastric artery was also prevented by Hoe‐140 (100 μ mol kg−1, i.v.). Gastric acid backdiffusion was evoked by perfusing the stomach with 15% ethanol, to break the gastric mucosal barrier, in the presence of luminal acid. Depending on the concentration of acid (0.05 and 0.15 M HC1), this procedure increased gastric mucosal blood flow by a factor of 1.6‐2.8 and caused formation of gross damage in 1.5‐3% of the glandular mucosa. Hoe‐140 (100 μmol kg−1, i.v.) failed to alter the moderate vasodilatation seen in the presence of 0.05 M HC1 but significantly (P < 0.05) attenuated the marked hyperaemia and enhanced the gross mucosal damage observed in the presence of 0.15 M HC1. These data show that bradykinin is able to enhance gastric mucosal blood flow via activation of B2 receptors. It appears as if this kinin is formed during severe acid challenge of the rat gastric mucosa and participates in the hyperaemic reaction to gastric acid backdiffusion. 1994 British Pharmacological Society

Original languageEnglish
Pages (from-to)1036-1042
Number of pages7
JournalBritish journal of pharmacology
Volume113
Issue number3
DOIs
Publication statusPublished - Nov 1994

Keywords

  • Bradykinin
  • Hoe‐140 (icatibant)
  • acid‐evoked gastric hyperaemia
  • bradykinin B receptors
  • bradykinin antagonist
  • gastric blood flow
  • gastric mucosal damage

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'Role of bradykinin in the hyperaemia following acid challenge of the rat gastric mucosa'. Together they form a unique fingerprint.

  • Cite this