Role of A2A adenosine receptors in regulation of opsonized E. coli-induced macrophage function

Balázs Csóka, Zoltán H. Németh, Zsolt Selmeczy, Balázs Koscsó, Pál Pacher, E. Vízi, Edwin A. Deitch, G. Haskó

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Adenosine is a biologically active molecule that is formed at sites of metabolic stress associated with trauma and inflammation, and its systemic level reaches high concentrations in sepsis. We have recently shown that inactivation of A2A adenosine receptors decreases bacterial burden as well as IL-10, IL-6, and MIP-2 production in mice that were made septic by cecal ligation and puncture (CLP). Macrophages are important in both elimination of pathogens and cytokine production in sepsis. Therefore, in the present study, we questioned whether macrophages are responsible for the decreased bacterial load and cytokine production in A2A receptor-inactivated septic mice. We showed that A2A KO and WT peritoneal macrophages obtained from septic animals were equally effective in phagocytosing opsonized E. coli. IL-10 production induced by opsonized E. coli was decreased in macrophages obtained from septic A2A KO mice as compared to WT counterparts. In contrast, the release of IL-6 and MIP-2 induced by opsonized E. coli was higher in septic A2A KO macrophages than WT macrophages. These results suggest that peritoneal macrophages are not responsible for the decreased bacterial load and diminished MIP-2 and IL-6 production that are observed in septic A2A KO mice. In contrast, peritoneal macrophages may contribute to the suppressive effect of A2A receptor inactivation on IL-10 production during sepsis.

Original languageEnglish
Pages (from-to)447-452
Number of pages6
JournalPurinergic Signalling
Volume3
Issue number4
DOIs
Publication statusPublished - Sep 2007

Fingerprint

Adenosine A2A Receptors
Macrophages
Escherichia coli
Peritoneal Macrophages
Interleukin-10
Interleukin-6
Sepsis
Bacterial Load
Cytokines
Physiological Stress
Punctures
Phagocytosis
Adenosine
Ligation
Inflammation
Wounds and Injuries

Keywords

  • Adenosine
  • Cytokines
  • Inflammation
  • Macrophages
  • Phagocytosis
  • Sepsis

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Cellular and Molecular Neuroscience

Cite this

Role of A2A adenosine receptors in regulation of opsonized E. coli-induced macrophage function. / Csóka, Balázs; Németh, Zoltán H.; Selmeczy, Zsolt; Koscsó, Balázs; Pacher, Pál; Vízi, E.; Deitch, Edwin A.; Haskó, G.

In: Purinergic Signalling, Vol. 3, No. 4, 09.2007, p. 447-452.

Research output: Contribution to journalArticle

Csóka, Balázs ; Németh, Zoltán H. ; Selmeczy, Zsolt ; Koscsó, Balázs ; Pacher, Pál ; Vízi, E. ; Deitch, Edwin A. ; Haskó, G. / Role of A2A adenosine receptors in regulation of opsonized E. coli-induced macrophage function. In: Purinergic Signalling. 2007 ; Vol. 3, No. 4. pp. 447-452.
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