Role of Altered Expression of MIR-146a, MIR-155, and MIR-122 in Pediatric Patients with Inflammatory Bowel Disease

Nóra J. Béres, Dolóresz Szabó, Dorottya Kocsis, Dániel Szucs, Zoltán Kiss, Katalin E. Müller, Gábor Lendvai, András Kiss, András Arató, Erna Sziksz, Ádám Vannay, Attila J. Szabó, Gábor Veres

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24 Citations (Scopus)

Abstract

Background: Evidence suggests the central role of tumor necrosis factor (TNF)-α in the pathomechanism of inflammatory bowel disease (IBD); however, its effect on epigenetic factors, including small non-coding microRNAs (miRs), is less known. Our present aim was the comparative investigation of the expression of TNF-α and immune response-related miRs in children with Crohn's disease (CD) and ulcerative colitis (UC). Methods: Fresh-frozen (FF) and formalin-fixed, paraffin-embedded (FFPE) biopsies were used to analyze the expression of miR-146a,-155,-122, and TNF-α by real-time reverse transcription polymerase chain reaction in macroscopically inflamed (CD: 12 FFPE and 24 FF; UC: 10 FF) and intact (CD: 12 FFPE; 14 FF) colonic biopsies of children with IBD and controls (16 FFPE; 23 FF). The expression of miR-146a,-155, and-122 was also determined in TNF-α-treated HT-29 colonic epithelial cells. Results: Increased expression of TNF-α was observed in the colonic mucosa of children with CD and UC in comparison with controls. Expression of miR-146a and-155 was higher in the inflamed mucosa of children with CD and UC than in the intact mucosa. Expression of miR-122 elevated in the macroscopically intact colonic regions of CD compared with controls and patients with UC. In HT-29 cells, TNF-α treatment increased the expression of miR-146a and-155, but not that of miR-122. Conclusions: Our results showed altered expression of miR-146a,-155, and-122 in the colonic mucosa of children with IBD and in TNF-α-treated colonic epithelial cells. Our data suggest the TNF-α-related involvement of these miRs in the pathogenesis of IBD.

Original languageEnglish
Pages (from-to)327-335
Number of pages9
JournalInflammatory bowel diseases
Volume22
Issue number2
DOIs
Publication statusPublished - Jan 11 2016

Keywords

  • Crohn's disease
  • MIR-122
  • MIR-146a
  • MIR-155
  • TNF-α
  • inflammatory bowel disease
  • microRNAs
  • pediatric
  • ulcerative colitis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Gastroenterology

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  • Cite this

    Béres, N. J., Szabó, D., Kocsis, D., Szucs, D., Kiss, Z., Müller, K. E., Lendvai, G., Kiss, A., Arató, A., Sziksz, E., Vannay, Á., Szabó, A. J., & Veres, G. (2016). Role of Altered Expression of MIR-146a, MIR-155, and MIR-122 in Pediatric Patients with Inflammatory Bowel Disease. Inflammatory bowel diseases, 22(2), 327-335. https://doi.org/10.1097/MIB.0000000000000687