Robertsonian translocations in wheat arise by centric misdivision of univalents at anaphase I and rejoining of broken centromeres during interkinesis of meiosis II

B. Friebe, P. Zhang, G. Linc, B. S. Gill

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Abstract

The mechanism of origin of Robertsonian translocations was investigated in plants monosomic for chromosome 1A of wheat and 1Ht of Elymus trachycaulus by GISH. Chromosomes 1A and 1Ht stayed univalent in all metaphase I cells analyzed, suggesting that Robertsonian translocations do not originate from meiotic recombination in centromeric regions with shared DNA sequence homology. At ana-/telophase I, the 1Ht and 1A univalents underwent either chromosome or chromatid segregation and misdivided in 6-7 % of the pollen mother cells. None of the ana-/telophases I analyzed had Robertsonian translocations, which were only observed in 2 % of the "half tetrads" at ana-/telophase II. The frequency of Robertsonian translocations observed at ana-/telophase II corresponds well with the number of Robertsonian translocations (1-4 %) detected in progenies derived from plants monosomic for group-1 chromosomes of wheat (1A, 1B, and 1D) and 1Ht of E. trachycaulus. Our data suggest that Robertsonian translocations arise from centric misdivision of univalents at ana-/telophase I, followed by segregation of the derived telocentric chromosomes to the same nucleus, and fusion of the broken ends during the ensuing interkinesis.

Original languageEnglish
Pages (from-to)293-297
Number of pages5
JournalCytogenetic and Genome Research
Volume109
Issue number1-3
DOIs
Publication statusPublished - 2005

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Telophase
Anaphase
Centromere
Meiosis
Triticum
Chromosomes
Elymus
Plant Chromosomes
Chromatids
Chromosomes, Human, Pair 1
Metaphase
Sequence Homology
Pollen
Genetic Recombination
Stem Cells

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

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title = "Robertsonian translocations in wheat arise by centric misdivision of univalents at anaphase I and rejoining of broken centromeres during interkinesis of meiosis II",
abstract = "The mechanism of origin of Robertsonian translocations was investigated in plants monosomic for chromosome 1A of wheat and 1Ht of Elymus trachycaulus by GISH. Chromosomes 1A and 1Ht stayed univalent in all metaphase I cells analyzed, suggesting that Robertsonian translocations do not originate from meiotic recombination in centromeric regions with shared DNA sequence homology. At ana-/telophase I, the 1Ht and 1A univalents underwent either chromosome or chromatid segregation and misdivided in 6-7 {\%} of the pollen mother cells. None of the ana-/telophases I analyzed had Robertsonian translocations, which were only observed in 2 {\%} of the {"}half tetrads{"} at ana-/telophase II. The frequency of Robertsonian translocations observed at ana-/telophase II corresponds well with the number of Robertsonian translocations (1-4 {\%}) detected in progenies derived from plants monosomic for group-1 chromosomes of wheat (1A, 1B, and 1D) and 1Ht of E. trachycaulus. Our data suggest that Robertsonian translocations arise from centric misdivision of univalents at ana-/telophase I, followed by segregation of the derived telocentric chromosomes to the same nucleus, and fusion of the broken ends during the ensuing interkinesis.",
author = "B. Friebe and P. Zhang and G. Linc and Gill, {B. S.}",
year = "2005",
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T1 - Robertsonian translocations in wheat arise by centric misdivision of univalents at anaphase I and rejoining of broken centromeres during interkinesis of meiosis II

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AU - Zhang, P.

AU - Linc, G.

AU - Gill, B. S.

PY - 2005

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N2 - The mechanism of origin of Robertsonian translocations was investigated in plants monosomic for chromosome 1A of wheat and 1Ht of Elymus trachycaulus by GISH. Chromosomes 1A and 1Ht stayed univalent in all metaphase I cells analyzed, suggesting that Robertsonian translocations do not originate from meiotic recombination in centromeric regions with shared DNA sequence homology. At ana-/telophase I, the 1Ht and 1A univalents underwent either chromosome or chromatid segregation and misdivided in 6-7 % of the pollen mother cells. None of the ana-/telophases I analyzed had Robertsonian translocations, which were only observed in 2 % of the "half tetrads" at ana-/telophase II. The frequency of Robertsonian translocations observed at ana-/telophase II corresponds well with the number of Robertsonian translocations (1-4 %) detected in progenies derived from plants monosomic for group-1 chromosomes of wheat (1A, 1B, and 1D) and 1Ht of E. trachycaulus. Our data suggest that Robertsonian translocations arise from centric misdivision of univalents at ana-/telophase I, followed by segregation of the derived telocentric chromosomes to the same nucleus, and fusion of the broken ends during the ensuing interkinesis.

AB - The mechanism of origin of Robertsonian translocations was investigated in plants monosomic for chromosome 1A of wheat and 1Ht of Elymus trachycaulus by GISH. Chromosomes 1A and 1Ht stayed univalent in all metaphase I cells analyzed, suggesting that Robertsonian translocations do not originate from meiotic recombination in centromeric regions with shared DNA sequence homology. At ana-/telophase I, the 1Ht and 1A univalents underwent either chromosome or chromatid segregation and misdivided in 6-7 % of the pollen mother cells. None of the ana-/telophases I analyzed had Robertsonian translocations, which were only observed in 2 % of the "half tetrads" at ana-/telophase II. The frequency of Robertsonian translocations observed at ana-/telophase II corresponds well with the number of Robertsonian translocations (1-4 %) detected in progenies derived from plants monosomic for group-1 chromosomes of wheat (1A, 1B, and 1D) and 1Ht of E. trachycaulus. Our data suggest that Robertsonian translocations arise from centric misdivision of univalents at ana-/telophase I, followed by segregation of the derived telocentric chromosomes to the same nucleus, and fusion of the broken ends during the ensuing interkinesis.

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