RNA processing: New mutants that affect endonucleolytic processing of RNA

Andras Miczak, Judy Ford, Micklos Marian, David Apirion

Research output: Contribution to journalArticle

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Abstract

A strain of Escherichiacoli carrying the rne-3071 mutation that affects the RNA processing enzyme ribonuclease E, was mutagenized, and double mutants deficient in RNA processing were isolated. The isolation was based on the appearance of a particular RNA precursor molecule upon infection of an rne mutant with a specific bacteriophage T4 deletion strain. From one of the double mutants the rne mutation was removed, and the new single mutant, designated rng, was examined. In this mutant the maturation of host RNA as well as of bacteriophage T4 RNA is affected. The effect of the rng mutation on RNA synthesis is unique and can be distinguished from the effects of the other established mutations in RNA processing. The effects of the rng mutation can be recognized invivo and invitro.

Original languageEnglish
Pages (from-to)690-698
Number of pages9
JournalBiochemical and biophysical research communications
Volume114
Issue number2
DOIs
Publication statusPublished - Jul 29 1983

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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