RNA polymerase III-transcribed EBER 1 and 2 transcription units are expressed and hypomethylated in the major Epstein-Barr virus-carrying cell types

J. Mináróvits, L. F. Hu, Z. Marcsek, S. Minarovits-Kormuta, G. Klein, I. Ernberg

Research output: Contribution to journalArticle

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Abstract

The genome of Epstein-Barr virus (EBV) codes for two non-translated small RNA molecules, EBER 1 and 2. We found that both EBERs are expressed in the major EBV-carrying cell types, group I and III Burkitt's lymphoma (BL) cell lines, lymphoblastoid cell lines (LCLs) and in two nude mouse-passaged nasopharyngeal carcinoma (NPC) tumours. The relative amount of EBER 1 and EBER 2 varied in different host cells but did not correlate with the cellular phenotype. The EBER coding and flanking sequences were predominantly hypomethylated at HpaII sites not only in LCLs which usually carry hypomethylated EBV genomes but also in BL and NPC cell lines harbouring EBV episomes that are highly methylated in other regions. Thus, the EBER trancription units, actively transcribed by RNA polymerase III in the major EBV-carrying cell types, represent a methylation-free region in the EBV genome similarly to regulatory sequences of the latent membrane protein gene when the latter is transcribed by RNA polymerase II.

Original languageEnglish
Pages (from-to)1687-1692
Number of pages6
JournalJournal of General Virology
Volume73
Issue number7
Publication statusPublished - 1992

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RNA Polymerase III
Human Herpesvirus 4
Cell Line
Burkitt Lymphoma
Genome
RNA Polymerase II
Nude Mice
Methylation
Epstein-Barr virus encoded RNA 2
Epstein-Barr virus encoded RNA 1
Membrane Proteins
Plasmids
RNA
Phenotype
Genes
Neoplasms

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

RNA polymerase III-transcribed EBER 1 and 2 transcription units are expressed and hypomethylated in the major Epstein-Barr virus-carrying cell types. / Mináróvits, J.; Hu, L. F.; Marcsek, Z.; Minarovits-Kormuta, S.; Klein, G.; Ernberg, I.

In: Journal of General Virology, Vol. 73, No. 7, 1992, p. 1687-1692.

Research output: Contribution to journalArticle

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AU - Klein, G.

AU - Ernberg, I.

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AB - The genome of Epstein-Barr virus (EBV) codes for two non-translated small RNA molecules, EBER 1 and 2. We found that both EBERs are expressed in the major EBV-carrying cell types, group I and III Burkitt's lymphoma (BL) cell lines, lymphoblastoid cell lines (LCLs) and in two nude mouse-passaged nasopharyngeal carcinoma (NPC) tumours. The relative amount of EBER 1 and EBER 2 varied in different host cells but did not correlate with the cellular phenotype. The EBER coding and flanking sequences were predominantly hypomethylated at HpaII sites not only in LCLs which usually carry hypomethylated EBV genomes but also in BL and NPC cell lines harbouring EBV episomes that are highly methylated in other regions. Thus, the EBER trancription units, actively transcribed by RNA polymerase III in the major EBV-carrying cell types, represent a methylation-free region in the EBV genome similarly to regulatory sequences of the latent membrane protein gene when the latter is transcribed by RNA polymerase II.

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