Rice flour as potential carbon source for production of phytase by Aspergillus niger F00735 strain

E. Bujna, F. Kukovics, Q. Nguyen, J. Rezessy-Szabó

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Production of phytase by Aspergillus niger F00735 strain in submerged fermentation was studied. The effects of various natural substrates with different phytate contents on secretion of extracellular phytase were investigated and the rice flour with about 5 mg g-1 of phytic acid was found to be the best one. The repression effect of high levels of phytic acid or inorganic phosphorous in fermentation medium (corn flour, wheat grit, soy flour, etc.) on production of phytase was also observed. The optimal concentration of rice flour as main carbon sourc e was determined in combination with sodium nitrate. The maximal activity (≈1500 U l-1, 1.5 times higher than using basal medium) was achieved on the 7th day in media containing 7.12% (w/v) rice flour and 0.86% (w/v) sodium nitrate. Supplementation of fermentation medium with different surfactants such as Tween series (20, 40, 60, 65, 80, 85) and Triton X-100 up to 0.1% (w/v) had no significant effects on the secretion of phytase enzyme, meanwhile at concentrations higher than 0.2% (w/v), decrease in enzyme activity was observed.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalActa Alimentaria
Volume42
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Nov 1 2013

Fingerprint

6-Phytase
rice flour
Aspergillus niger
phytases
Flour
Carbon
phytic acid
Phytic Acid
sodium nitrate
carbon
Fermentation
fermentation
secretion
submerged fermentation
corn flour
soy flour
surfactants
Polysorbates
Octoxynol
Enzymes

Keywords

  • fermentation
  • optimization
  • phytase
  • rice flour
  • RSM
  • surfactant

ASJC Scopus subject areas

  • Food Science

Cite this

Rice flour as potential carbon source for production of phytase by Aspergillus niger F00735 strain. / Bujna, E.; Kukovics, F.; Nguyen, Q.; Rezessy-Szabó, J.

In: Acta Alimentaria, Vol. 42, No. SUPPL. 1, 01.11.2013, p. 1-9.

Research output: Contribution to journalArticle

Bujna, E. ; Kukovics, F. ; Nguyen, Q. ; Rezessy-Szabó, J. / Rice flour as potential carbon source for production of phytase by Aspergillus niger F00735 strain. In: Acta Alimentaria. 2013 ; Vol. 42, No. SUPPL. 1. pp. 1-9.
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