Rice bran as a substrate for proteolytic enzyme production

Alagarsamy Sumantha, Paul Deepa, Chandran Sandhya, George Szakacs, Carlos Ricardo Soccol, Ashok Pandey

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31 Citations (Scopus)


Rice bran was used as the substrate for screening nine strains of Rhizopus sp. for neutral protease production by solid-state fermentation. The best producer, Rhizopus microsporus NRRL 3671, was used for optimizing the process parameters for enzyme production. Fermentation carried out with 44.44 % initial moisture content at a temperature of 30°C for 72 h was found to be the optimum for enzyme secretion by the fermenting organism. While most of the carbon supplements favored enzyme production, addition of casein resulted in a marginal increase in protease yield. Fermentation was then carried out under optimized conditions to obtain the crude extract of the enzyme, which was partially purified by precipitation and dialysis. A 3-fold increase in the enzyme purity was achieved in this manner. The enzyme was found to be a metalloprotease, being activated by Mn2+, with maximal activity at a temperature of 60°C and pH 7.0.

Original languageEnglish
Pages (from-to)843-851
Number of pages9
JournalBrazilian Archives of Biology and Technology
Issue number5
Publication statusPublished - Sep 2006


  • Agro-industrial residues
  • Neutral protease
  • Rhizopus
  • Rice bran
  • Solid-state fermentation

ASJC Scopus subject areas

  • General

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