Ribotoxin genes in isolates of Aspergillus section Clavati

J. Varga, Robert A. Samson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Ribotoxins are ribosome inactivator proteins with high specificity against the sarcin/ricin domain of the 28S ribosomal RNA. We examined the presence of ribotoxin genes in isolates of species recently assigned to Aspergillus section Clavati using specific primer pairs. All species assigned to this section have been found to carry ribotoxin genes. Phylogenetic analysis of the sequences of the amplified gene fragments allowed us to classify the genes to different groups including the α-sarcin, gigantin, c-sarcin and mitogillin/restrictocin families. Two species, A. longivesica and N. acanthosporus produced ribotoxins which were only distantly related to gigantins and c-sarcins, respectively. Comparison of the protein sequences of the genes to known ribotoxin sequences revealed that all of them carry the presumed catalytic residues of ribotoxins, the cystein residues, and also the two Trp residues of α-sarcin conserved in all ribotoxins known so far. These data indicate that these genes probably encode active ribotoxins. Further studies are in progress to examine the secretion and activities of these new ribotoxins.

Original languageEnglish
Pages (from-to)481-485
Number of pages5
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume94
Issue number3
DOIs
Publication statusPublished - 2008

Fingerprint

Aspergillus
Genes
28S Ribosomal RNA
Ricin
Ribosomes
Sequence Analysis
Proteins
gigantin

Keywords

  • Antifungal peptides
  • Aspergillus section Clavati
  • Clavin
  • Gigantin
  • Mitogillin
  • Phylogenetic analysis
  • Restrictocin
  • Ribotoxins

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Ribotoxin genes in isolates of Aspergillus section Clavati. / Varga, J.; Samson, Robert A.

In: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, Vol. 94, No. 3, 2008, p. 481-485.

Research output: Contribution to journalArticle

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