Rhodococcus sovatensis sp. Nov., an actinomycete isolated from the hypersaline and heliothermal Lake Ursu

András Táncsics, István Máthé, Tibor Benedek, Erika M. Tóth, Ewelina Atasayar, Cathrin Spröer, Károly Márialigeti, Tamás Felföldi, Balázs Kriszt

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A Gram-stain-positive, strictly aerobic, mesophilic bacterium, designated H004T, was isolated from a water sample of the hypersaline and heliothermal Lake Ursu, Sovata, Romania. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H004T formed a distinct phyletic lineage within the genus Rhodococcus. It shared the highest 16S rRNA gene sequence similarity with Rhodococcus yunnanensis YIM 70056T (98.80%), followed by Rhodococcus fascians LMG 3623T (98.73%), Rhodococcus cercidiphylli YIM 65003T (98.73%), Rhodococcus cerastii C5T (98.58%) and Rhodococcus kyotonensis DS472T (98.53%). The alkB-based phylogenetic analysis further confirmed that this strain constitutes a highly unique lineage within the genus. Chemotaxonomic characteristics, including the predominant fatty acids acids C15:0, C18:1ω 9c, C19:1ω 11c/ C19:1ω9c and C16:1ω7c/iso-C15:0 2-OH, the major quinone MK-8(H2), the presence of mycolic acids and cell-wall chemotype IV were also consistent with the properties of members of the genus Rhodococcus. The DNA G+C content of strain H004T was 65.4 mol%. The results of DNA–DNA hybridization analyses with the closest relatives, in combination with the alkB-based phylogenetic analysis, as well as the chemotaxonomic and physiological data, demonstrated that isolate H004T represents a novel species of the genus Rhodococcus, for which the name Rhodococcus sovatensissp. nov. is proposed. The type strain is H004T (=DSM 102881T=NCAIM B.02632T).

Original languageEnglish
Article number001514
Pages (from-to)190-196
Number of pages7
JournalInternational journal of systematic and evolutionary microbiology
Volume67
Issue number2
DOIs
Publication statusPublished - Feb 2017

Keywords

  • AlkB
  • Alkane 1-monooxygenase
  • Heliothermal
  • Hypersaline
  • Rhodococcus

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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