A Gram-stain-positive, strictly aerobic, mesophilic bacterium, designated H004T, was isolated from a water sample of the hypersaline and heliothermal Lake Ursu, Sovata, Romania. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H004T formed a distinct phyletic lineage within the genus Rhodococcus. It shared the highest 16S rRNA gene sequence similarity with Rhodococcus yunnanensis YIM 70056T (98.80%), followed by Rhodococcus fascians LMG 3623T (98.73%), Rhodococcus cercidiphylli YIM 65003T (98.73%), Rhodococcus cerastii C5T (98.58%) and Rhodococcus kyotonensis DS472T (98.53%). The alkB-based phylogenetic analysis further confirmed that this strain constitutes a highly unique lineage within the genus. Chemotaxonomic characteristics, including the predominant fatty acids acids C15:0, C18:1ω 9c, C19:1ω 11c/ C19:1ω9c and C16:1ω7c/iso-C15:0 2-OH, the major quinone MK-8(H2), the presence of mycolic acids and cell-wall chemotype IV were also consistent with the properties of members of the genus Rhodococcus. The DNA G+C content of strain H004T was 65.4 mol%. The results of DNA–DNA hybridization analyses with the closest relatives, in combination with the alkB-based phylogenetic analysis, as well as the chemotaxonomic and physiological data, demonstrated that isolate H004T represents a novel species of the genus Rhodococcus, for which the name Rhodococcus sovatensissp. nov. is proposed. The type strain is H004T (=DSM 102881T=NCAIM B.02632T).
|Number of pages||7|
|Journal||International journal of systematic and evolutionary microbiology|
|Publication status||Published - Feb 2017|
- Alkane 1-monooxygenase
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics