Rhodamine backfilling and confocal microscopy as a tool for the unambiguous identification of neuronal cell types: A study of the neurones of the rat cochlear nucleus

Krisztina Pocsai, Balázs Pál, Pál Pap, Gábor Bakondi, Lívia Kosztka, Zoltán Rusznák, Géza Szucs

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Adequate interpretation of the functional data characterising the projection neurones of the cochlear nucleus (CN) is impossible without the unequivocal classification of these cell types at the end of the experiments. In this study, morphological criteria applicable for unambiguous identification of CN neurones have been sought. The neurones were labelled with rhodamine from incisions severing the projection pathways of the individual cell types, allowing their selective labelling and morphological characterisation. Confocal microscopy was employed for the investigation of the rhodamine-filled cells whose morphology was assessed after reconstructing the three-dimensional images of the cell bodies and proximal processes. The diameters of the somata and the number of processes originating from the cell bodies were also determined. In most of the cases, unambiguous identification of the bushy, octopus and Purkinje-like cells was relatively straightforward. On the other hand, precise classification of the pyramidal cells was often difficult, especially because giant cells could easily possess morphological features resembling pyramidal neurones. Occasionally, giant cells also mimicked the appearance of octopus neurones, which may be another important source of identification error, especially as these two cell types are often situated close to each other in the CN. It is concluded that morphological criteria defined in the present work may be effectively applied for the unambiguous identification of the projection neurones of the CN, even following functional measurements, when the correct cell classification is essential for the interpretation of the experimental data. Moreover, the present study also confirmed that Purkinje-like cells project to the cerebellum.

Original languageEnglish
Pages (from-to)529-538
Number of pages10
JournalBrain Research Bulletin
Volume71
Issue number5
DOIs
Publication statusPublished - Mar 15 2007

Keywords

  • Cochlear nucleus
  • Confocal microscopy
  • Fluorescence microscopy
  • Purkinje-like cell
  • Retrograde labelling
  • Rhodamine

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Rhodamine backfilling and confocal microscopy as a tool for the unambiguous identification of neuronal cell types: A study of the neurones of the rat cochlear nucleus'. Together they form a unique fingerprint.

  • Cite this