Rhizobium meliloti produces a family of sulfated lipo-oligosaccharides exhibiting different degrees of plant host specificity

M. Schultze, B. Quiclet-Sire, É. Kondorosi, H. Virelizier, J. N. Glushka, G. Endré, S. D. Gero, A. Kondorosi

Research output: Contribution to journalArticle

185 Citations (Scopus)

Abstract

We have shown that a Rhizobium meliloti strain overexpressing nodulation genes excreted high amounts of a family of N-acylated and 6-O-sulfated N- acetyl-β-1,4-D-glucosamine penta-, tetra-, and trisaccharide Nod factors. Either a C(16:2) or a C(16:3) acyl chain is attached to the nonreducing end subunit, whereas the sulfate group is bound to the reducing glucosamine. One of the tetrasaccharides is identical to the previously described NodRm-1 factor. The two pentasaccharides as well as NodRm-1 were purified and tested for biological activity. In the root hair deformation assay the pentasaccharides show similar activities on the host plants Medicago sativa and Melilotus albus and on the non-host plant Vicia sativa at a dilution of up to 0.01-0.001 μM, in contrast to NodRm-1, which displays a much higher specific activity for Medicago and Melilotus than for Vicia. The active concentration range of the pentasaccharides is more narrow on Medicago than on Melilotus and Vicia. In addition to root hair deformation, the different Nod factors were shown to induce nodule formation on M. sativa. We suggest that the production of a series of active signal molecules with different degrees of specificity might be important in controlling the symbiosis of R. meliloti with several different host plants or under different environmental conditions.

Original languageEnglish
Pages (from-to)192-196
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number1
Publication statusPublished - 1992

Fingerprint

Melilotus
Sinorhizobium meliloti
Host Specificity
Oligosaccharides
Vicia
Medicago
Medicago sativa
Glucosamine
Vicia sativa
Trisaccharides
Rubiaceae
Symbiosis
Sulfates
Genes

Keywords

  • Medicago sativa
  • nod genes
  • nodulation
  • plant growth regulation
  • symbiosis

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Rhizobium meliloti produces a family of sulfated lipo-oligosaccharides exhibiting different degrees of plant host specificity. / Schultze, M.; Quiclet-Sire, B.; Kondorosi, É.; Virelizier, H.; Glushka, J. N.; Endré, G.; Gero, S. D.; Kondorosi, A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 1, 1992, p. 192-196.

Research output: Contribution to journalArticle

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