Rewiring of RSK–PDZ Interactome by Linear Motif Phosphorylation

Gergő Gógl, Beáta Biri-Kovács, Fabien Durbesson, Pau Jane, Yves Nomine, Camille Kostmann, Viktória Bilics, Márton Simon, A. Reményi, Renaud Vincentelli, Gilles Trave, L. Nyitray

Research output: Contribution to journalArticle

Abstract

Phosphorylation of short linear peptide motifs is a widespread process for the dynamic regulation of protein–protein interactions. However, the global impact of phosphorylation events on the protein–protein interactome is rarely addressed. The disordered C-terminal tail of ribosomal S6 kinase 1 (RSK1) binds to PDZ domain-containing scaffold proteins, and it harbors a phosphorylatable PDZ-binding motif (PBM) responsive to epidermal growth factor stimulation. Here, we examined binding of two versions of the RSK1 PBM, either phosphorylated or unphosphorylated at position − 3, to almost all (95%) of the 266 PDZ domains of the human proteome. PBM phosphorylation dramatically altered the PDZ domain-binding landscape of RSK1, by strengthening or weakening numerous interactions to various degrees. The RSK–PDZome interactome analyzed in this study reveals how linear motif-based phospho-switches convey stimulus-dependent changes in the context of related network components.

Original languageEnglish
JournalJournal of molecular biology
DOIs
Publication statusPublished - Jan 1 2019

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Ribosomal Protein S6 Kinases
PDZ Domains
Phosphorylation
Proteome
Epidermal Growth Factor
Tail
Peptides
Proteins

Keywords

  • MAPK
  • PDZ
  • phosphorylation
  • protein–protein interaction
  • RSK

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Rewiring of RSK–PDZ Interactome by Linear Motif Phosphorylation. / Gógl, Gergő; Biri-Kovács, Beáta; Durbesson, Fabien; Jane, Pau; Nomine, Yves; Kostmann, Camille; Bilics, Viktória; Simon, Márton; Reményi, A.; Vincentelli, Renaud; Trave, Gilles; Nyitray, L.

In: Journal of molecular biology, 01.01.2019.

Research output: Contribution to journalArticle

Gógl, G, Biri-Kovács, B, Durbesson, F, Jane, P, Nomine, Y, Kostmann, C, Bilics, V, Simon, M, Reményi, A, Vincentelli, R, Trave, G & Nyitray, L 2019, 'Rewiring of RSK–PDZ Interactome by Linear Motif Phosphorylation', Journal of molecular biology. https://doi.org/10.1016/j.jmb.2019.01.038
Gógl G, Biri-Kovács B, Durbesson F, Jane P, Nomine Y, Kostmann C et al. Rewiring of RSK–PDZ Interactome by Linear Motif Phosphorylation. Journal of molecular biology. 2019 Jan 1. https://doi.org/10.1016/j.jmb.2019.01.038
Gógl, Gergő ; Biri-Kovács, Beáta ; Durbesson, Fabien ; Jane, Pau ; Nomine, Yves ; Kostmann, Camille ; Bilics, Viktória ; Simon, Márton ; Reményi, A. ; Vincentelli, Renaud ; Trave, Gilles ; Nyitray, L. / Rewiring of RSK–PDZ Interactome by Linear Motif Phosphorylation. In: Journal of molecular biology. 2019.
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