Revival of calcium-binding proteins for neuromorphology: Secretagogin typifies distinct cell populations in the avian brain

Georgina Gáti, Dávid Lendvai, Tomas Hökfelt, Tibor Harkany, A. Alpár

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In the vertebrate nervous system, the Ca2+-binding proteins parvalbumin, calbindin and calretinin have been extensively used to elaborate the molecular diversity of neuronal subtypes. Secretagogin is a phylogenetically conserved Ca2+-binding protein, which marks neuronal populations largely distinct from other Ca2+-binding proteins in mammals. Whether secretagogin is expressed in nonmammalian vertebrates, particularly in birds, and, if so, with a brain cytoarchitectonic design different from that of mammals is unknown. Here, we show that secretagogin is already present in the hatchlings' brain with continued presence into adulthood. Secretagogin- immunoreactive neurons primarily accumulate in the olfactory bulb, septum, subpallial amygdala, hippocampus, hypothalamus, habenular nuclei and deep layers of the optic tectum of adult domestic chicks (Gallus domesticus). In the olfactory bulb, secretagogin labels periglomerular neurons as well as a cell continuum ascending dorsomedially, reaching the ventricular wall. Between the hippocampus and septal nuclei, the interconnecting thin septal tissue harbors secretagogin-immunoreactive neurons that contact the ventricular wall with their ramifying dendritic processes. Secretagogin is also present in the neuroendocrine hypothalamus, with particularly rich neuronal clusters seen in its suprachiasmatic and infundibular nuclei. Secretagogin expression identified a hitherto undescribed cell contingent along intratelencephalic cell-free laminae separating brain regions or marking the palliosubpallial boundary, as well as a dense neuronal population in the area corticoidea lateralis. In both the telencephalon and midbrain, secretagogin complemented the distribution of the canonical 'neuronal' Ca2+-binding proteins. Our findings identify novel neuronal subtypes, connectivity patterns in brain areas functionally relevant to olfaction, orientation, behavior as well as endocrine functions, which will help refine existing concepts on the neuronal diversity and organizational principles of the avian brain.

Original languageEnglish
Pages (from-to)82-92
Number of pages11
JournalBrain, Behavior and Evolution
Volume83
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Calcium-Binding Proteins
Carrier Proteins
Brain
Population
Olfactory Bulb
Neurons
Hypothalamus
Vertebrates
Mammals
Hippocampus
Habenula
Calbindin 2
Calbindins
Parvalbumins
Telencephalon
Septal Nuclei
Arcuate Nucleus of Hypothalamus
Suprachiasmatic Nucleus
Superior Colliculi
Smell

Keywords

  • Comparative neuroanatomy
  • Cortex
  • Evolution
  • Pallium

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Behavioral Neuroscience
  • Medicine(all)

Cite this

Revival of calcium-binding proteins for neuromorphology : Secretagogin typifies distinct cell populations in the avian brain. / Gáti, Georgina; Lendvai, Dávid; Hökfelt, Tomas; Harkany, Tibor; Alpár, A.

In: Brain, Behavior and Evolution, Vol. 83, No. 2, 2014, p. 82-92.

Research output: Contribution to journalArticle

Gáti, Georgina ; Lendvai, Dávid ; Hökfelt, Tomas ; Harkany, Tibor ; Alpár, A. / Revival of calcium-binding proteins for neuromorphology : Secretagogin typifies distinct cell populations in the avian brain. In: Brain, Behavior and Evolution. 2014 ; Vol. 83, No. 2. pp. 82-92.
@article{f82e1febc74740b9a9f5c8ac72776ef1,
title = "Revival of calcium-binding proteins for neuromorphology: Secretagogin typifies distinct cell populations in the avian brain",
abstract = "In the vertebrate nervous system, the Ca2+-binding proteins parvalbumin, calbindin and calretinin have been extensively used to elaborate the molecular diversity of neuronal subtypes. Secretagogin is a phylogenetically conserved Ca2+-binding protein, which marks neuronal populations largely distinct from other Ca2+-binding proteins in mammals. Whether secretagogin is expressed in nonmammalian vertebrates, particularly in birds, and, if so, with a brain cytoarchitectonic design different from that of mammals is unknown. Here, we show that secretagogin is already present in the hatchlings' brain with continued presence into adulthood. Secretagogin- immunoreactive neurons primarily accumulate in the olfactory bulb, septum, subpallial amygdala, hippocampus, hypothalamus, habenular nuclei and deep layers of the optic tectum of adult domestic chicks (Gallus domesticus). In the olfactory bulb, secretagogin labels periglomerular neurons as well as a cell continuum ascending dorsomedially, reaching the ventricular wall. Between the hippocampus and septal nuclei, the interconnecting thin septal tissue harbors secretagogin-immunoreactive neurons that contact the ventricular wall with their ramifying dendritic processes. Secretagogin is also present in the neuroendocrine hypothalamus, with particularly rich neuronal clusters seen in its suprachiasmatic and infundibular nuclei. Secretagogin expression identified a hitherto undescribed cell contingent along intratelencephalic cell-free laminae separating brain regions or marking the palliosubpallial boundary, as well as a dense neuronal population in the area corticoidea lateralis. In both the telencephalon and midbrain, secretagogin complemented the distribution of the canonical 'neuronal' Ca2+-binding proteins. Our findings identify novel neuronal subtypes, connectivity patterns in brain areas functionally relevant to olfaction, orientation, behavior as well as endocrine functions, which will help refine existing concepts on the neuronal diversity and organizational principles of the avian brain.",
keywords = "Comparative neuroanatomy, Cortex, Evolution, Pallium",
author = "Georgina G{\'a}ti and D{\'a}vid Lendvai and Tomas H{\"o}kfelt and Tibor Harkany and A. Alp{\'a}r",
year = "2014",
doi = "10.1159/000357834",
language = "English",
volume = "83",
pages = "82--92",
journal = "Brain, Behavior and Evolution",
issn = "0006-8977",
publisher = "S. Karger AG",
number = "2",

}

TY - JOUR

T1 - Revival of calcium-binding proteins for neuromorphology

T2 - Secretagogin typifies distinct cell populations in the avian brain

AU - Gáti, Georgina

AU - Lendvai, Dávid

AU - Hökfelt, Tomas

AU - Harkany, Tibor

AU - Alpár, A.

PY - 2014

Y1 - 2014

N2 - In the vertebrate nervous system, the Ca2+-binding proteins parvalbumin, calbindin and calretinin have been extensively used to elaborate the molecular diversity of neuronal subtypes. Secretagogin is a phylogenetically conserved Ca2+-binding protein, which marks neuronal populations largely distinct from other Ca2+-binding proteins in mammals. Whether secretagogin is expressed in nonmammalian vertebrates, particularly in birds, and, if so, with a brain cytoarchitectonic design different from that of mammals is unknown. Here, we show that secretagogin is already present in the hatchlings' brain with continued presence into adulthood. Secretagogin- immunoreactive neurons primarily accumulate in the olfactory bulb, septum, subpallial amygdala, hippocampus, hypothalamus, habenular nuclei and deep layers of the optic tectum of adult domestic chicks (Gallus domesticus). In the olfactory bulb, secretagogin labels periglomerular neurons as well as a cell continuum ascending dorsomedially, reaching the ventricular wall. Between the hippocampus and septal nuclei, the interconnecting thin septal tissue harbors secretagogin-immunoreactive neurons that contact the ventricular wall with their ramifying dendritic processes. Secretagogin is also present in the neuroendocrine hypothalamus, with particularly rich neuronal clusters seen in its suprachiasmatic and infundibular nuclei. Secretagogin expression identified a hitherto undescribed cell contingent along intratelencephalic cell-free laminae separating brain regions or marking the palliosubpallial boundary, as well as a dense neuronal population in the area corticoidea lateralis. In both the telencephalon and midbrain, secretagogin complemented the distribution of the canonical 'neuronal' Ca2+-binding proteins. Our findings identify novel neuronal subtypes, connectivity patterns in brain areas functionally relevant to olfaction, orientation, behavior as well as endocrine functions, which will help refine existing concepts on the neuronal diversity and organizational principles of the avian brain.

AB - In the vertebrate nervous system, the Ca2+-binding proteins parvalbumin, calbindin and calretinin have been extensively used to elaborate the molecular diversity of neuronal subtypes. Secretagogin is a phylogenetically conserved Ca2+-binding protein, which marks neuronal populations largely distinct from other Ca2+-binding proteins in mammals. Whether secretagogin is expressed in nonmammalian vertebrates, particularly in birds, and, if so, with a brain cytoarchitectonic design different from that of mammals is unknown. Here, we show that secretagogin is already present in the hatchlings' brain with continued presence into adulthood. Secretagogin- immunoreactive neurons primarily accumulate in the olfactory bulb, septum, subpallial amygdala, hippocampus, hypothalamus, habenular nuclei and deep layers of the optic tectum of adult domestic chicks (Gallus domesticus). In the olfactory bulb, secretagogin labels periglomerular neurons as well as a cell continuum ascending dorsomedially, reaching the ventricular wall. Between the hippocampus and septal nuclei, the interconnecting thin septal tissue harbors secretagogin-immunoreactive neurons that contact the ventricular wall with their ramifying dendritic processes. Secretagogin is also present in the neuroendocrine hypothalamus, with particularly rich neuronal clusters seen in its suprachiasmatic and infundibular nuclei. Secretagogin expression identified a hitherto undescribed cell contingent along intratelencephalic cell-free laminae separating brain regions or marking the palliosubpallial boundary, as well as a dense neuronal population in the area corticoidea lateralis. In both the telencephalon and midbrain, secretagogin complemented the distribution of the canonical 'neuronal' Ca2+-binding proteins. Our findings identify novel neuronal subtypes, connectivity patterns in brain areas functionally relevant to olfaction, orientation, behavior as well as endocrine functions, which will help refine existing concepts on the neuronal diversity and organizational principles of the avian brain.

KW - Comparative neuroanatomy

KW - Cortex

KW - Evolution

KW - Pallium

UR - http://www.scopus.com/inward/record.url?scp=84899889674&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899889674&partnerID=8YFLogxK

U2 - 10.1159/000357834

DO - 10.1159/000357834

M3 - Article

C2 - 24776989

AN - SCOPUS:84899889674

VL - 83

SP - 82

EP - 92

JO - Brain, Behavior and Evolution

JF - Brain, Behavior and Evolution

SN - 0006-8977

IS - 2

ER -