Revisiting CB1 receptor as drug target in human melanoma

I. Kenessey, Balázs Bánki, Ágnes Márk, Norbert Varga, J. Tóvári, A. Ladányi, E. Rásó, J. Tímár

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Previous studies have indicated the antitumoral effect of human melanocytes, human melanoma cell lines expressing CB1 receptor (CB1), and of the peritumoral administration of endocannabinoids. In the present study, we systematically screened several human melanoma cell lines for the expression of CNR1 and demonstrated transcription of the authentic gene. The product of CNR1, the CB1 protein, was found localized to the cell membrane as well as to the cytoskeleton. Further, the studied human melanoma cell lines expressed functional CB1 since physiological and synthetic ligands, anandamide (AEA), Met- F-AEA, ACEA and AM251 showed a wide range of biological effects in vitro, for example anti-proliferative, proapoptotic and anti-migratory. More importantly, our studies revealed that systemic administration of a stable CB1 agonist, ACEA, into SCID mice specifically inhibited liver colonization of human melanoma cells. Since therapeutic options for melanoma patients are still very limited, the endocannabinoid-CB1 receptor system may offer a novel target.

Original languageEnglish
Pages (from-to)857-866
Number of pages10
JournalPathology and Oncology Research
Volume18
Issue number4
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Cannabinoid Receptor CB1
Melanoma
Endocannabinoids
Pharmaceutical Preparations
Cell Line
SCID Mice
Melanocytes
Cytoskeleton
Cell Membrane
Ligands
Liver
Genes
Proteins

Keywords

  • CB1 receptor
  • Endocannabinoid
  • Human malignant melanoma
  • Metastasis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Revisiting CB1 receptor as drug target in human melanoma. / Kenessey, I.; Bánki, Balázs; Márk, Ágnes; Varga, Norbert; Tóvári, J.; Ladányi, A.; Rásó, E.; Tímár, J.

In: Pathology and Oncology Research, Vol. 18, No. 4, 10.2012, p. 857-866.

Research output: Contribution to journalArticle

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