Revising mtDNA haplotypes of the ancient Hungarian conquerors with next generation sequencing

Endre Neparáczki, Klaudia Kocsy, Gábor Endre Tóth, Zoltán Maróti, Tibor Kalmár, Péter Bihari, István Nagy, György Pálfi, Erika Molnár, István Raskó, Tibor Török

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

As part of the effort to create a high resolution representative sequence database of the medieval Hungarian conquerors we have resequenced the entire mtDNA genome of 24 published ancient samples with Next Generation Sequencing, whose haplotypes had been previously determined with traditional PCR based methods. We show that PCR based methods are prone to erroneous haplotype or haplogroup determination due to ambiguous sequence reads, and many of the resequenced samples had been classified inaccurately. The SNaPshot method applied with published ancient DNA authenticity criteria is the most straightforward and cheapest PCR based approach for testing a large number of coding region SNP-s, which greatly facilitates correct haplogroup determination.

Original languageEnglish
Article numbere0174886
JournalPloS one
Volume12
Issue number4
DOIs
Publication statusPublished - Apr 2017

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Neparáczki, E., Kocsy, K., Tóth, G. E., Maróti, Z., Kalmár, T., Bihari, P., Nagy, I., Pálfi, G., Molnár, E., Raskó, I., & Török, T. (2017). Revising mtDNA haplotypes of the ancient Hungarian conquerors with next generation sequencing. PloS one, 12(4), [e0174886]. https://doi.org/10.1371/journal.pone.0174886