Revising lysenin expression of earthworm coelomocytes

Balázs Opper, András Bognár, Diána Heidt, P. Németh, P. Engelmann

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Lysenin is a species-specific bioactive molecule of Eisenia andrei earthworms. This protein is a potent antimicrobial factor; however its cellular expression and induction against pathogens are still not fully understood. We developed a novel monoclonal antibody against lysenin and applied this molecular tool to characterize its production and antimicrobial function. We demonstrated by flow cytometry and immunocytochemistry that one subgroup of earthworm immune cells (so called coelomocytes), the chloragocytes expressed the highest amount of lysenin. Then, we compared lysenin expression with earlier established coelomocyte (EFCC) markers. In addition, we determined by immunohistology of earthworm tissues that lysenin production is only restricted to free-floating chloragocytes. Moreover, we observed that upon in vitro Staphylococcus aureus but not Escherichia coli challenged coelomocytes over-expressed and then secreted lysenin. These results indicate that among subpopulations of coelomocytes, lysenin is mainly produced by chloragocytes and its expression can be modulated by Gram-positive bacterial exposure.

Original languageEnglish
Pages (from-to)214-218
Number of pages5
JournalDevelopmental and Comparative Immunology
Volume39
Issue number3
DOIs
Publication statusPublished - Mar 2013

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Oligochaeta
lysenin
Staphylococcus aureus
Flow Cytometry
Immunohistochemistry
Monoclonal Antibodies
Escherichia coli

Keywords

  • Antimicrobial activity
  • Coelomocytes
  • Innate immunity
  • Monoclonal antibodies

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

Revising lysenin expression of earthworm coelomocytes. / Opper, Balázs; Bognár, András; Heidt, Diána; Németh, P.; Engelmann, P.

In: Developmental and Comparative Immunology, Vol. 39, No. 3, 03.2013, p. 214-218.

Research output: Contribution to journalArticle

Opper, Balázs ; Bognár, András ; Heidt, Diána ; Németh, P. ; Engelmann, P. / Revising lysenin expression of earthworm coelomocytes. In: Developmental and Comparative Immunology. 2013 ; Vol. 39, No. 3. pp. 214-218.
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