Reversed-phased thin-layer chromatography used to study the simultaneous effect of pH and ion strength on the lipophilicity of chlorphexidine

T. Cserháti, Maria Szögyi, László Lelkes

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The effect of ion strength and pH value of the eluent on the determination of the lipophilicity of chlorhexidine was studied by reversed-phase thin-layer chromatography. The method has been improved by using various buffers: aqueous solutions of formic, acetic and propionic acids and their sodium salts in different ratios and in various concentration. Stepwise regression analysis separated the effect of pH value, ion strength and acid type on the lipophilicity of chlorhexidine and proved that the ion strength exerted a higer impact than the pH value did. The effect of alkyl chain length of the acids was of secondary importance.

Original languageEnglish
Pages (from-to)263-270
Number of pages8
JournalJournal of Biochemical and Biophysical Methods
Volume16
Issue number4
DOIs
Publication statusPublished - 1988

Fingerprint

Thin layer chromatography
Thin Layer Chromatography
Chlorhexidine
Ions
Formates
Acids
Propionates
Reverse-Phase Chromatography
Chain length
Regression analysis
Acetic Acid
Buffers
Salts
Sodium
Regression Analysis

Keywords

  • Chlorhexidine
  • Lipophilic character
  • Reversed-phase thin-layer chromatography
  • Stepwise regression analysis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

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T1 - Reversed-phased thin-layer chromatography used to study the simultaneous effect of pH and ion strength on the lipophilicity of chlorphexidine

AU - Cserháti, T.

AU - Szögyi, Maria

AU - Lelkes, László

PY - 1988

Y1 - 1988

N2 - The effect of ion strength and pH value of the eluent on the determination of the lipophilicity of chlorhexidine was studied by reversed-phase thin-layer chromatography. The method has been improved by using various buffers: aqueous solutions of formic, acetic and propionic acids and their sodium salts in different ratios and in various concentration. Stepwise regression analysis separated the effect of pH value, ion strength and acid type on the lipophilicity of chlorhexidine and proved that the ion strength exerted a higer impact than the pH value did. The effect of alkyl chain length of the acids was of secondary importance.

AB - The effect of ion strength and pH value of the eluent on the determination of the lipophilicity of chlorhexidine was studied by reversed-phase thin-layer chromatography. The method has been improved by using various buffers: aqueous solutions of formic, acetic and propionic acids and their sodium salts in different ratios and in various concentration. Stepwise regression analysis separated the effect of pH value, ion strength and acid type on the lipophilicity of chlorhexidine and proved that the ion strength exerted a higer impact than the pH value did. The effect of alkyl chain length of the acids was of secondary importance.

KW - Chlorhexidine

KW - Lipophilic character

KW - Reversed-phase thin-layer chromatography

KW - Stepwise regression analysis

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