Reversed-phase liquid chromatography for the separation of chlorpromazine, imipramine and some of their metabolites

J. Fekete, P. Del Castilho, J. C. Kraak

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Reversed-phase liquid chromatography on a C18 bonded silica with water-alcohol mixtures containing decylamine as the mobile phase was investigated for its applicability to the separation of chlorpromazine, imipramine and their metabolites. The addition of decylamine to the mobile phase was found to be necessary in order to obtain symmetrical peaks. The influence of the decylamine and ethanol concentrations and of the pH of the mobile phase was investigated and these parameters were found to be useful for the adjustment of the retention. The phase system developed was found to be suitable for the required separation. The phase system was applied to the analysis of imipramine and its metabolites, desmethylimipramine and didesmethylimipramine, and desmethylchlorpromazine in human plasma.

Original languageEnglish
Pages (from-to)319-327
Number of pages9
JournalJournal of Chromatography A
Volume204
Issue numberC
DOIs
Publication statusPublished - Jan 16 1981

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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