Reversal of multidrug resistance of cancer cells in vitro: Modification of drug resistance by selected carotenoids

N. Gyémánt, M. Tanaka, Péter Molnár, J. Deli, László Mándoky, J. Molnár

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The development of multidrug resistance (MDR) causes difficulties in the chemotherapy of of human cancer. Investigation of the possibility of reversal of MDR has been greatly aided by the use of cell lines with aquired resitance to anticancer agents in vitro or transfected with the mdr1 gene. The aim of this study was to examine new perspectives of chemotherapy focused on natural, carotenoid compounds, in connection with the modification of MDR. The function of the MDR protein was examined via the R123 drug accumulation of both cell lines in the presence of carotenoids. The fluorescence of the cell population was measured by flow cytometry. The most effective resistance modifiers Monoepoxy-b-carotene, (5S, 8S)-capsochrome, (8'S) Luteoxanthin, (9Z)-Violaxanthin, (9Z)-Zeaxanthin, (13Z)-Zeaxanthin were assayed for their antiproliferative effects in combination with the anti-cancer drug epirubicin. (13Z)-Zeaxanthin was able to enhance the antiproliferative effect on human mdr1 gene transfected mouse lymphoma and anthracycline resistant human breast cancer cell line MCF7. (8'S)-luteoxanthin, (5S, 8S)-capsochrome and (9Z)-zeaxanthin treatment revealed synergism with epirubicin on resistant mouse lymphoma. The enhanced antiproliferative activity of epirubicin combinated with (9Z)-Violaxanthin was more significant on MCF7 cells resistant to anthracycline.

Original languageEnglish
Pages (from-to)367-374
Number of pages8
JournalAnticancer Research
Volume26
Issue number1 A
Publication statusPublished - Jan 2006

Fingerprint

Multiple Drug Resistance
Carotenoids
Drug Resistance
Epirubicin
Anthracyclines
Cell Line
Lymphoma
Neoplasms
P-Glycoproteins
Drug Therapy
MCF-7 Cells
Pharmaceutical Preparations
Antineoplastic Agents
Genes
Flow Cytometry
Fluorescence
Breast Neoplasms
Zeaxanthins
In Vitro Techniques
Population

Keywords

  • Cancer cells
  • Carotenoids
  • Multidrug resistance

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Reversal of multidrug resistance of cancer cells in vitro : Modification of drug resistance by selected carotenoids. / Gyémánt, N.; Tanaka, M.; Molnár, Péter; Deli, J.; Mándoky, László; Molnár, J.

In: Anticancer Research, Vol. 26, No. 1 A, 01.2006, p. 367-374.

Research output: Contribution to journalArticle

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