Revealing the Involvement of Extended Hydrogen Bond Networks in the Cooperative Function between Distant Sites in Bacterial Reaction Centers

Julia Tandori, Laura Baciou, Emil Alexov, Péter Maróti, Marianne Schiffer, Deborah K. Hanson, Pierre Sebban

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In reaction center proteins of photosynthetic bacteria, the amplitude of proton uptake induced by the one-electron reduction of either of the two quinone electron acceptors (QA and QB) is an intrinsic observable of the electrostatic interactions associated with the redox function of the complex. We report here that, in Rhodobacter capsulatus, complete restoration of proton uptake (upon formation of QA - and QB -) to the level found in the wild type is observed in a mutant reaction center in which a tyrosine substitution in the QA environment (AlaM274 → Tyr) is coupled with mutations of acidic residues near QB (GluL212 → Ala/AspL213 → Ala) that initially cancel the proton uptake above pH 8. This result demonstrates that proton uptake occurs by strong cooperation between structural motifs, such as hydrogen-bonded networks, that span the 18 Å distance between the two quinone acceptors.

Original languageEnglish
Pages (from-to)45513-45515
Number of pages3
JournalJournal of Biological Chemistry
Volume276
Issue number49
DOIs
Publication statusPublished - Dec 7 2001

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Protons
Hydrogen
Hydrogen bonds
Electrons
Rhodobacter capsulatus
Photosynthetic Reaction Center Complex Proteins
Coulomb interactions
Static Electricity
Restoration
Oxidation-Reduction
Tyrosine
Bacteria
Substitution reactions
Mutation
Proteins
benzoquinone

ASJC Scopus subject areas

  • Biochemistry

Cite this

Revealing the Involvement of Extended Hydrogen Bond Networks in the Cooperative Function between Distant Sites in Bacterial Reaction Centers. / Tandori, Julia; Baciou, Laura; Alexov, Emil; Maróti, Péter; Schiffer, Marianne; Hanson, Deborah K.; Sebban, Pierre.

In: Journal of Biological Chemistry, Vol. 276, No. 49, 07.12.2001, p. 45513-45515.

Research output: Contribution to journalArticle

Tandori, Julia ; Baciou, Laura ; Alexov, Emil ; Maróti, Péter ; Schiffer, Marianne ; Hanson, Deborah K. ; Sebban, Pierre. / Revealing the Involvement of Extended Hydrogen Bond Networks in the Cooperative Function between Distant Sites in Bacterial Reaction Centers. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 49. pp. 45513-45515.
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