Revascularization decreases 8-isoprostaglandin F excretion in chronic lower limb ischemia

P. Rossi, A. Riutta, P. Kuukasjärvi, T. Vehmas, I. Muchá, J. P. Salenius

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

8-Isoprostaglandin F is one of a series of isoprostanes formed by free radical catalysed peroxidation of arachidonic acid. Urinary 8-isoprostaglandin F is a new marker which reflects oxidative stress in vivo and can be utilized as a diagnostic tool to assess the extent of oxidative stress in various disease states associated with lipid peroxidation. Increased levels of 8-isoprostaglandin F in cardiac ischemia/reperfusion provide evidence for oxidative stress during coronary perfusion. In animal studies, the restoration of blood flow after lower limb ischemia is followed by reperfusion syndrome. In this study we investigated whether lower limb ischemia/reperfusion is associated with oxidative stress, as reflected by urinary levels of 8-isoprostaglandin F. Ten patients (mean age 72 years, range 61-82 years) suffering from chronic lower limb ischemia and 10 healthy volunteers (mean age 69 years, range 60-79 years) participated in the study. In all patients, diagnostic angiography had revealed stenosis or occlusion either in the aortoiliac or femoropopliteal region. Surgical revascularization consisted of femoropopliteal reconstruction, femorofemoral reconstruction, aortobifemorial reconstruction, or femoral endartectomy. Urine samples from patients were collected a day before surgery and in the second postoperative day. Urinary 8-isoprostaglandin F was extracted on a C2 silica cartridge and determinated by radioimmunoassay. After revascularization, 8-isoprostaglandin F excretion (pg/μmol creatinine, mean±SD) was decreased by 2.5-fold (preoperative 48.9±8.9, postoperative 19.1±9.5, P2α, chronic lower limb ischemia is associated with increased oxidative stress, which is decreased by revascularization.

Original languageEnglish
Pages (from-to)97-101
Number of pages5
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume71
Issue number2
DOIs
Publication statusPublished - Aug 2004

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Oxidative stress
Lower Extremity
Oxidative Stress
Ischemia
Reperfusion
Isoprostanes
Angiography
Thigh
Ambulatory Surgical Procedures
Arachidonic Acid
Silicon Dioxide
Surgery
Lipid Peroxidation
Restoration
Free Radicals
Radioimmunoassay
Creatinine
Healthy Volunteers
Pathologic Constriction
Animals

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Revascularization decreases 8-isoprostaglandin F excretion in chronic lower limb ischemia. / Rossi, P.; Riutta, A.; Kuukasjärvi, P.; Vehmas, T.; Muchá, I.; Salenius, J. P.

In: Prostaglandins Leukotrienes and Essential Fatty Acids, Vol. 71, No. 2, 08.2004, p. 97-101.

Research output: Contribution to journalArticle

Rossi, P. ; Riutta, A. ; Kuukasjärvi, P. ; Vehmas, T. ; Muchá, I. ; Salenius, J. P. / Revascularization decreases 8-isoprostaglandin F excretion in chronic lower limb ischemia. In: Prostaglandins Leukotrienes and Essential Fatty Acids. 2004 ; Vol. 71, No. 2. pp. 97-101.
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