Reusability of immunoaffinity columns for determination of fumonisins in maize

B. Fazekas, Andrea Koncz-Tar, Edit Tóth-Hajdu, Melinda Zomborszky-Kovács

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Eighteen maize samples were assayed for fumonisin B1 (FB1) and B2 content by immunoaffinity column coupled with high performance liquid chromatography (HPLC). The FumoniTest columns were used once for the isolation of fumonisins (single-use column method). In the second part of the assay the columns were regenerated. After elution with methanol, PBS solution was left on the column for one day at room temperature to regenerate the columns (regenerated column method). The efficiency of columns regenerated twice was tested by determining FB1 recovery and the reproducibility of the determinations. The recovery rate of FB1 proved to be 82% by the single-use column method (RSD: 5.7%) and 82.6% (RSD: 5.6%) by the regenerated column method; 500-8000 ng FB1 loaded onto the columns did not affect column performances. Nearly identical values were obtained when the FB1 content of fumonisin-containing maize samples was determined by both methods. The results indicate that the FumoniTest columns can be regenerated by the method applied at least twice without decrease in column performance. The fumonisin affinity, capacity and specificity of the regenerated columns were not changed. Thus, columns regenerated in this way can be used for determining the fumonisin content of maize samples at least three times.

Original languageEnglish
Pages (from-to)259-263
Number of pages5
JournalNatural Toxins
Volume7
Issue number6
DOIs
Publication statusPublished - 1999

Fingerprint

Fumonisins
Reusability
Zea mays
Recovery
High performance liquid chromatography
Methanol
Assays
fumonisin B1
High Pressure Liquid Chromatography
Temperature

Keywords

  • Fumonisin
  • Immunoaffinity column
  • Regeneration

ASJC Scopus subject areas

  • Toxicology

Cite this

Reusability of immunoaffinity columns for determination of fumonisins in maize. / Fazekas, B.; Koncz-Tar, Andrea; Tóth-Hajdu, Edit; Zomborszky-Kovács, Melinda.

In: Natural Toxins, Vol. 7, No. 6, 1999, p. 259-263.

Research output: Contribution to journalArticle

Fazekas, B. ; Koncz-Tar, Andrea ; Tóth-Hajdu, Edit ; Zomborszky-Kovács, Melinda. / Reusability of immunoaffinity columns for determination of fumonisins in maize. In: Natural Toxins. 1999 ; Vol. 7, No. 6. pp. 259-263.
@article{159fe3fa6b5d403698cfa0b3a7d48595,
title = "Reusability of immunoaffinity columns for determination of fumonisins in maize",
abstract = "Eighteen maize samples were assayed for fumonisin B1 (FB1) and B2 content by immunoaffinity column coupled with high performance liquid chromatography (HPLC). The FumoniTest columns were used once for the isolation of fumonisins (single-use column method). In the second part of the assay the columns were regenerated. After elution with methanol, PBS solution was left on the column for one day at room temperature to regenerate the columns (regenerated column method). The efficiency of columns regenerated twice was tested by determining FB1 recovery and the reproducibility of the determinations. The recovery rate of FB1 proved to be 82{\%} by the single-use column method (RSD: 5.7{\%}) and 82.6{\%} (RSD: 5.6{\%}) by the regenerated column method; 500-8000 ng FB1 loaded onto the columns did not affect column performances. Nearly identical values were obtained when the FB1 content of fumonisin-containing maize samples was determined by both methods. The results indicate that the FumoniTest columns can be regenerated by the method applied at least twice without decrease in column performance. The fumonisin affinity, capacity and specificity of the regenerated columns were not changed. Thus, columns regenerated in this way can be used for determining the fumonisin content of maize samples at least three times.",
keywords = "Fumonisin, Immunoaffinity column, Regeneration",
author = "B. Fazekas and Andrea Koncz-Tar and Edit T{\'o}th-Hajdu and Melinda Zomborszky-Kov{\'a}cs",
year = "1999",
doi = "10.1002/1522-7189(199911/12)7:6<259::AID-NT67>3.0.CO;2-P",
language = "English",
volume = "7",
pages = "259--263",
journal = "Natural Toxins",
issn = "1056-9014",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

TY - JOUR

T1 - Reusability of immunoaffinity columns for determination of fumonisins in maize

AU - Fazekas, B.

AU - Koncz-Tar, Andrea

AU - Tóth-Hajdu, Edit

AU - Zomborszky-Kovács, Melinda

PY - 1999

Y1 - 1999

N2 - Eighteen maize samples were assayed for fumonisin B1 (FB1) and B2 content by immunoaffinity column coupled with high performance liquid chromatography (HPLC). The FumoniTest columns were used once for the isolation of fumonisins (single-use column method). In the second part of the assay the columns were regenerated. After elution with methanol, PBS solution was left on the column for one day at room temperature to regenerate the columns (regenerated column method). The efficiency of columns regenerated twice was tested by determining FB1 recovery and the reproducibility of the determinations. The recovery rate of FB1 proved to be 82% by the single-use column method (RSD: 5.7%) and 82.6% (RSD: 5.6%) by the regenerated column method; 500-8000 ng FB1 loaded onto the columns did not affect column performances. Nearly identical values were obtained when the FB1 content of fumonisin-containing maize samples was determined by both methods. The results indicate that the FumoniTest columns can be regenerated by the method applied at least twice without decrease in column performance. The fumonisin affinity, capacity and specificity of the regenerated columns were not changed. Thus, columns regenerated in this way can be used for determining the fumonisin content of maize samples at least three times.

AB - Eighteen maize samples were assayed for fumonisin B1 (FB1) and B2 content by immunoaffinity column coupled with high performance liquid chromatography (HPLC). The FumoniTest columns were used once for the isolation of fumonisins (single-use column method). In the second part of the assay the columns were regenerated. After elution with methanol, PBS solution was left on the column for one day at room temperature to regenerate the columns (regenerated column method). The efficiency of columns regenerated twice was tested by determining FB1 recovery and the reproducibility of the determinations. The recovery rate of FB1 proved to be 82% by the single-use column method (RSD: 5.7%) and 82.6% (RSD: 5.6%) by the regenerated column method; 500-8000 ng FB1 loaded onto the columns did not affect column performances. Nearly identical values were obtained when the FB1 content of fumonisin-containing maize samples was determined by both methods. The results indicate that the FumoniTest columns can be regenerated by the method applied at least twice without decrease in column performance. The fumonisin affinity, capacity and specificity of the regenerated columns were not changed. Thus, columns regenerated in this way can be used for determining the fumonisin content of maize samples at least three times.

KW - Fumonisin

KW - Immunoaffinity column

KW - Regeneration

UR - http://www.scopus.com/inward/record.url?scp=0033286136&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033286136&partnerID=8YFLogxK

U2 - 10.1002/1522-7189(199911/12)7:6<259::AID-NT67>3.0.CO;2-P

DO - 10.1002/1522-7189(199911/12)7:6<259::AID-NT67>3.0.CO;2-P

M3 - Article

C2 - 11122516

AN - SCOPUS:0033286136

VL - 7

SP - 259

EP - 263

JO - Natural Toxins

JF - Natural Toxins

SN - 1056-9014

IS - 6

ER -