Retromer Ensures the Degradation of Autophagic Cargo by Maintaining Lysosome Function in Drosophila

Tamás Maruzs, Péter Lorincz, Zsuzsanna Szatmári, Szilvia Széplaki, Zoltán Sándor, Zsolt Lakatos, Gina Puska, G. Juhász, M. Sass

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The retromer is an evolutionarily conserved coat complex that consists of Vps26, Vps29, Vps35 and a heterodimer of sorting nexin (Snx) proteins in yeast. Retromer mediates the recycling of transmembrane proteins from endosomes to the trans-Golgi network, including receptors that are essential for the delivery of hydrolytic enzymes to lysosomes. Besides its function in lysosomal enzyme receptor recycling, involvement of retromer has also been proposed in a variety of vesicular trafficking events, including early steps of autophagy and endocytosis. Here we show that the late stages of autophagy and endocytosis are impaired in Vps26 and Vps35 deficient Drosophila larval fat body cells, but formation of autophagosomes and endosomes is not compromised. Accumulation of aberrant autolysosomes and amphisomes in the absence of retromer function appears to be the consequence of decreased degradative capacity, as they contain undigested cytoplasmic material. Accordingly, we show that retromer is required for proper cathepsin L trafficking mainly independent of LERP, the Drosophila homolog of the cation-independent mannose 6-phosphate receptor. Finally, we find that Snx3 and Snx6 are also required for proper autolysosomal degradation in Drosophila larval fat body cells. In this study we characterize the role of the retromer complex in autophagy and endocytosis in Drosophila larval fat body cells.

Original languageEnglish
Pages (from-to)1088-1107
Number of pages20
JournalTraffic
Volume16
Issue number10
DOIs
Publication statusPublished - Oct 1 2015

Fingerprint

Lysosomes
Drosophila
Fat Body
Autophagy
Endocytosis
Fats
Adipocytes
Degradation
Recycling
Endosomes
Sorting Nexins
IGF Type 2 Receptor
Cathepsin L
Enzymes
trans-Golgi Network
Yeast
Fungal Proteins
Cations
Proteins

Keywords

  • Autophagy
  • Drosophila
  • Endocytosis
  • Lysosomal storage diseases
  • Lysosome
  • Retromer

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Structural Biology
  • Molecular Biology
  • Genetics

Cite this

Maruzs, T., Lorincz, P., Szatmári, Z., Széplaki, S., Sándor, Z., Lakatos, Z., ... Sass, M. (2015). Retromer Ensures the Degradation of Autophagic Cargo by Maintaining Lysosome Function in Drosophila. Traffic, 16(10), 1088-1107. https://doi.org/10.1111/tra.12309

Retromer Ensures the Degradation of Autophagic Cargo by Maintaining Lysosome Function in Drosophila. / Maruzs, Tamás; Lorincz, Péter; Szatmári, Zsuzsanna; Széplaki, Szilvia; Sándor, Zoltán; Lakatos, Zsolt; Puska, Gina; Juhász, G.; Sass, M.

In: Traffic, Vol. 16, No. 10, 01.10.2015, p. 1088-1107.

Research output: Contribution to journalArticle

Maruzs, T, Lorincz, P, Szatmári, Z, Széplaki, S, Sándor, Z, Lakatos, Z, Puska, G, Juhász, G & Sass, M 2015, 'Retromer Ensures the Degradation of Autophagic Cargo by Maintaining Lysosome Function in Drosophila', Traffic, vol. 16, no. 10, pp. 1088-1107. https://doi.org/10.1111/tra.12309
Maruzs T, Lorincz P, Szatmári Z, Széplaki S, Sándor Z, Lakatos Z et al. Retromer Ensures the Degradation of Autophagic Cargo by Maintaining Lysosome Function in Drosophila. Traffic. 2015 Oct 1;16(10):1088-1107. https://doi.org/10.1111/tra.12309
Maruzs, Tamás ; Lorincz, Péter ; Szatmári, Zsuzsanna ; Széplaki, Szilvia ; Sándor, Zoltán ; Lakatos, Zsolt ; Puska, Gina ; Juhász, G. ; Sass, M. / Retromer Ensures the Degradation of Autophagic Cargo by Maintaining Lysosome Function in Drosophila. In: Traffic. 2015 ; Vol. 16, No. 10. pp. 1088-1107.
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